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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 1755-1762
DOI: https://doi.org/10.20546/ijcmas.2018.712.204


Interferon Stimulated Gene 15 and Myxovirus Resistance 2 Genes are Upregulated during Early Pregnancy in Buffaloes
B.S. Pradeep Nag1, N. Arunmozhi2*, T. Sarath2, S.A. Asokan2, K. Vijayarani3, G. Monica2, D. Gopikrishnan2 and K. Krishnakumar2
1National Dairy Research Institute, Karnal, India
2Department of Veterinary Gynaecology and Obstetrics, 3Department of Animal Biotechnology, MVC, Chennai, India
*Corresponding author
Abstract:

The present study was done with the objective of finding the differential expression of ISG 15 and MX 2 genes in pregnant and non pregnant heifer and pleuriparous buffaloes. A total of 40 heifer and pleuriparous buffaloes were selected and divided into 2 experiments, Experiment I consisted of heifers (Group I and Group II) and Experiment II (Group III and Group IV) consisted of pleuiparous buffaloes. Group I and group III were considered as non inseminated control groups and group II and IVas treatment groups. Blood collection was done on days 0, 14, 16, 18, 20 and 25 and peripheral blood mononuclear cells (PBMC) were isolated from which cDNA was synthesised for expression studies. Expression of ISG 15 and MX 2 genes were carried out by quantitative real-time PCR, relative expression was calculated by 2-ΔΔCT using GAPDH as housekeeping gene. Both the genes were upregulated between days 14 to 20, reaching the peak by day 20 and decreased on day 25 to the values similar to day 14. A significantly (P≤0.01) higher relative expression of both ISG 15 and MX 2 genes was observed on days 18 and 20 post oestrum, in both the experimental groups.


Keywords: Interferon stimulated gene 15, Myxovirus resistance 2, Heifer, Pleuriparous buffalo, Upregulated
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How to cite this article:

Pradeep Nag, B.S., N. Arunmozhi, T. Sarath, S.A. Asokan, K. Vijayarani, G. Monica, D. Gopikrishnan and Krishnakumar, K. 2018. Interferon Stimulated Gene 15 and Myxovirus Resistance 2 Genes are Upregulated during Early Pregnancy in Buffaloes.Int.J.Curr.Microbiol.App.Sci. 7(12): 1755-1762. doi: https://doi.org/10.20546/ijcmas.2018.712.204