National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was done with the objective of finding the differential expression of ISG 15 and MX 2 genes in pregnant and non pregnant heifer and pleuriparous buffaloes. A total of 40 heifer and pleuriparous buffaloes were selected and divided into 2 experiments, Experiment I consisted of heifers (Group I and Group II) and Experiment II (Group III and Group IV) consisted of pleuiparous buffaloes. Group I and group III were considered as non inseminated control groups and group II and IVas treatment groups. Blood collection was done on days 0, 14, 16, 18, 20 and 25 and peripheral blood mononuclear cells (PBMC) were isolated from which cDNA was synthesised for expression studies. Expression of ISG 15 and MX 2 genes were carried out by quantitative real-time PCR, relative expression was calculated by 2-ΔΔCT using GAPDH as housekeeping gene. Both the genes were upregulated between days 14 to 20, reaching the peak by day 20 and decreased on day 25 to the values similar to day 14. A significantly (P≤0.01) higher relative expression of both ISG 15 and MX 2 genes was observed on days 18 and 20 post oestrum, in both the experimental groups.
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