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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 1748-1754
DOI: https://doi.org/10.20546/ijcmas.2018.712.203


Evaluation of Antagonistic Potential of Biocontrol Agents against Macrophomina Phaseolina (tassi) Goid. causing Stem and Root Rot of Sesame [Sesamum Indicum l.] under In vitro and In vivo
Ashish Kumar Satpathi* and N.M. Gohel
Department of Plant Pathology, B. A. College of Agriculture, Anand Agricultural University, Anand, Gujarat, India
*Corresponding author
Abstract:

Effect of eight antagonists viz., Trichoderma atroviride, T. harzianum, T. virens, T. fasciculatum, T. asperellum, T. viride, Pseudomonas fluorescens and Bacillus subtilis were studied for their antagonism against Macrophomina phaseolina by dual culture method and six fungicides viz. Azoxystrobin 23 SC, Carbendazim (12%) + Mancozeb (63%) 75 WP, Carbendazim (25%) + Mancozeb (50%) 75 WS were evaluated at two different concentrations (500 ppm and 1000 ppm) by poisoned food technique against the same pathogen. Out of eight antagonists evaluated, Trichoderma atroviride showed strong antagonistic effect against the pathogen with highest growth inhibition (60.00%) and among the six fungicides, carbendazim (12%) + mancozeb (63%), carboxin (37.5%) + thiram (37.5%), carbendazim (25%) + mancozeb (50%) and carbendazim were proved to be effective with cent per cent growth inhibition of the pathogen at both the concentrations (500 and 1000 ppm) tested. The effective biocontrol agents and chemicals which were found promising under laboratory studies were further evaluated for the management of stem and root rot of sesame under pot conditions. The highest germination (100%), higher shoot (17.06 cm) and root (4.03 cm) length, vigour index (2032) and minimum plant mortality (8.22%) was achieved through treatment T4 i.e. seed treatment with T. harzianum (2×108cfu/g) AAU isolate @ 10 g/ kg seeds + soil application of T. harzianum (2×108cfu/g) enriched FYM @ 100g/pot.


Keywords: Sesame, Stem and root rot, Macrophomina phaseolina, Antagonists, Fungicides, Dual culture, Poisoned food technique, Management
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How to cite this article:

Ashish Kumar Satpathi and Gohel, N.M. 2018. Evaluation of Antagonistic Potential of Biocontrol Agents against Macrophomina Phaseolina (tassi) Goid. causing Stem and Root Rot of Sesame [Sesamum Indicum l.] under In vitro and In vivo.Int.J.Curr.Microbiol.App.Sci. 7(12): 1748-1754. doi: https://doi.org/10.20546/ijcmas.2018.712.203