Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 1642-1655
DOI: https://doi.org/10.20546/ijcmas.2018.712.192


Analysis of Somaclonal Variation using Molecular Markers in in vitro Regenerated Chrysanthemum cv. Pusa Centenary
M. Jayanthi1, Rajesh Gupta1, P.K. Mandal2* and Kanchan B.M. Singh1
1Division of Nematology, Indian Agricultural Research Institute, New Delhi - 110012, India
2National Research Centre for Plant Biotechnology, New Delhi-110012, India
*Corresponding author
Abstract:

Detection of somaclonal variation in tissue cultured plants is essential for conservation of elite varieties and also to avoid undesired characters. The genetic fidelity of in vitro regenerated Chrysanthemum cv. Pusa centenary, a commercially important cultivar was assessed using RAPD and ISSR markers. Regeneration was obtained via organogenesis and somatic embryogenesis from leaf and petal explants respectively. Murashige and Skoog (MS) media with different plant growth regulators (PGR) were used for in vitro experiments with leaf explants. Among the different PGR combinations used in MS media, 2,4-Dichlorophenoxyacetic acid (2,4-D) (1 mg L-1) and á¼€-naphthalene acetic acid (NAA) (1 mg L-1) along with 0.5 mg L-1of 6-benzylaminopurine (BAP) or Kinetin (Kin) was optimal for producing 83-93% callus induction from leaf explants. On transfer to light on MS media with BAP (0.5 mg L-1) and reduced auxins, the calli started turning green and proliferating into plantlets. Petal explants on the same media, produced direct somatic embryos within 10-14 days. On subculture, profuse production of somatic embryos with root and shoot poles was obtained. Nineteen primers (9 RAPD and 10 ISSR primers) produced 825 clear reproducible and scorable bands. While RAPD primers produced completely monomorphic bands with both leaf and petal regenerated plants in comparison to their mother plant, it was found that ISSR primers were able to detect variation in leaf callus derived plantlets. The plants regenerated via organogenesis from leaf explants were showing a variation of 0.66% with ISSR primers and plants regenerated from distinct somatic embryos of petal explants showed completely monomorphic bands with both RAPD and ISSR proving it’s true to type nature. These results indicate that in vitro culture process induces rearrangements at the DNA level and demonstrates discrepancies between the pathways involved in regeneration.


Keywords: Callus induction, Direct embryogenesis, Genetic fidelity, RAPD and ISSR
Download this article as Download

How to cite this article:

Jayanthi, M., Rajesh Gupta, P.K. Mandal and Kanchan B.M. Singh. 2018. Analysis of Somaclonal Variation using Molecular Markers in in vitro Regenerated Chrysanthemum cv. Pusa Centenary.Int.J.Curr.Microbiol.App.Sci. 7(12): 1642-1655. doi: https://doi.org/10.20546/ijcmas.2018.712.192