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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 1510-1514
DOI: https://doi.org/10.20546/ijcmas.2018.712.178


Fluorescein Diacetate (FDA): Measure of Total Microbial Activity and as Indicator of Soil Quality
N.P. Navnage1 and R.M. Shinde2*
1Department of Soil Science and Agricultural Chemistry
2Department of Plant Molecular Biology and Biotechnology, Indira Gandhi Krishi Vishwavidyalaya,Raipur 492012 (C.G.), India
*Corresponding author
Abstract:

Soil is crucial to life on earth. Enormous agriculture practices may emerge a numerous problem like reduction in soil fertility, erosion, ground water contamination which has direct impact on the soil health. It is indispensable to study overall microbial activity potential and different changes in soil biological properties for maintaining soil health and its quality. Soil enzyme is essential to play important role in maintaining soil health and to asses it quality. The fluorescein diacetate (FDA) hydrolysis is one of the important to measures the enzyme activity of microbial populations and can provide an assessment of microbial activity in soil. Assessment of Hydrolysis of fluorescein diacetate [3′, 6′-diacetylfluorescein (FDA)] has been suggested as prospective method for determination of total microbial activity because it includes several enzyme classes including lipases, esterases, and proteases. Spectrophotometric determination of the hydrolysis of fluorescein diacetate (FDA) was shown to be a simple, sensitive, and rapid method for determining microbial activity in soil. The assessment of FDA in soil is done by incubating the soil sample with buffer and FDA for 1 – 2 hours. The amount of fluorescent color formation during the incubation is indicative of the enzymatic activity of the microbial community in the sample. The intensity of the color will be measured by a spectrophotometer and compared to a standard curve to determine the relative microbial activity in your sample. The main intendment of this review paper to define the role of FDA hydrolysis in soil environment as well as method to estimate FDA enzyme activity in soil. Paper also illustrate that FDA enzyme activity serves as microbial indicator and measure of total microbial activity to asses soil health and is quality.


Keywords: Fluorescein Diacetate (FDA), Role, Microbial indicator, Methods
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How to cite this article:

Navnage, N.P. and Shinde, R.M. 2018. Fluorescein Diacetate (FDA): Measure of Total Microbial Activity and as Indicator of Soil Quality.Int.J.Curr.Microbiol.App.Sci. 7(12): 1510-1514. doi: https://doi.org/10.20546/ijcmas.2018.712.178