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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 508-514
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.059


A Study on Etiological Agents with Special Reference to Fungal Isolates causing Chronic Suppurative Otitis Media in a Tertiary Care Hospital
Vrutika V. Gandhi*, P. Neelu Sree and M. Kalyani
Saveetha Medical College and Hospital, Thandalam, Kanchipuram District, India
*Corresponding author
Abstract:

Chronic suppurative otitis media is a condition characterized by inflammation of middle ear affecting tympanic membrane, middle ear mucosa and other middle ear structures. To isolate, identify and characterize the fungal organisms and to detect the antifungal susceptibility pattern of the isolates by using conventional microbiological methods from specimens of clinically proved cases of chronic suppurative otitis media. Two hundred and forty five patients with CSOM were studied. Swabs were collected from each patient and cultured for bacteria and fungus.  Standard methods for isolation and identification of bacteria and fungus were followed.  Antibiotic susceptibility testing was done by Kirby-Bauer’s disc diffusion method. Antifungal susceptibility testing was done by Disc Diffusion and Microbroth Dilution method. Out of the 245 samples studied for bacterial and fungal isolates, 225 samples showed  growth of pathogens, 20 samples did not show any growth, 201 samples showed bacterial growth, The most predominant organism was Staphylococcus aureus, 24 samples showed fungal growth, Aspergillus sps being the predominant isolate. Antifungal susceptibility testing was also performed with discs fluconazole (10mcg) and itraconazole (10mcg) 23 isolates showed sensitivity to both and one resistant isolate was subjected to Minimum inhibitory concentration  by Microbroth dilution method. Patients would be benefitted by accurate diagnosis of type of infection and the appropriate drug that will be sensitive for the infection can be administered to them.


Keywords: Chronic suppurative otitis media, Minimum inhibitory concentration, Aspergillus species.
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How to cite this article:

Vrutika V. Gandhi, P. Neelu Sree and M. Kalyani. 2016. A Study on Etiological Agents with Special Reference to Fungal Isolates causing Chronic Suppurative Otitis Media in a Tertiary Care HospitalInt.J.Curr.Microbiol.App.Sci. 5(11): 508-514. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.059