Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 1233-1255
DOI: https://doi.org/10.20546/ijcmas.2018.712.154


Cloning and Over Expression of CVS Rabies Virus Glycoprotein Gene in Pichia pastoris by Multimeriztion
T.M. Ningaraju1* and P.H. Ramanjini Gowda2
1Department of Biotechnology, College of Sericulture, Chintamani, UAS(B), Karnataka, India
2Department of Biotechnology, University of Agricultural Sciences, Bangalore, Karnataka, India
*Corresponding author
Abstract:

Rabies is an endemic, fatal zoonotic disease which is transmitted by the bite of an infected animal. The glycoprotein of rabies virus is most antigenic and immunogenic determinant present in rabies virion and can serve as effective target for development of vaccine. The CVS rabies glycoprotein gene (1575 bp) was cloned into cloning vector pTZ57R/T and confirmed by PCR and Sequencing, further the glycoprotein gene was sub cloned into yeast integrative vector pPICZαA for expression and over expressed by using pPIC9K vector. Linearised recombinant vector containing glycoprotein gene was introduced into Pichia pastoris (GS115 and X-33) by electrophoration and the recombinant yeast clones were identified by PCR analysis. The recombinant protein expressed in yeast was confirmed by SDS-PAGE, ELISA, western blot and over expression was confirmed RT-PCR. These results allow us to conclude that P. pastoris is a convenient system for expression as well as for over expression of CVS rabies glycoprotein through multimeriation.


Keywords: Glycoprotein gene; Pichia pastoris; CVS Rabies Virus, SDS-PAGE: RT-PCR
Download this article as Download

How to cite this article:

Ningaraju, T.M. and Ramanjini Gowda, P.H. 2018. Cloning and Over Expression of CVS Rabies Virus Glycoprotein Gene in Pichia pastoris by Multimeriztion.Int.J.Curr.Microbiol.App.Sci. 7(12): 1233-1255. doi: https://doi.org/10.20546/ijcmas.2018.712.154