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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:12, December, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 1177-1183
DOI: https://doi.org/10.20546/ijcmas.2018.712.146


Effect of Osmotic Stress on the Organic Acid Production Profile and Tricalcium Phosphate Solubilization by Osmotolerant Bacteria
Gurupadam Hema Bindu1, Govindan Selvakumar1*, Kaushal K. Upreti1, Narayana Sunil Kumarand Duraisamy Kalaivanan1
1ICAR- Indian Institute of Horticultural Research, Hesaraghatta Lake Post Bengaluru-560089, India
2 Gokaraju Rangaraju Institute of Engineering and Technology, Hyderabad, India
*Corresponding author
Abstract:

Osmotolerant Plant Growth Promoting Rhizobacteria (PGPR) plays an important role in plant growth promotion in water stressed environments. Since phosphate solubilisation is one of the major mechanisms of plant growth promotion under water stressed conditions, this study was conducted to determine the effect of osmotic stress on the organic acid production profile and phosphate solubilization abilities of twelve osmotolerant bacterial isolates in NBRIP medium supplemented with 25% PEG 8000 (-1.97 MPa). It was observed that the imposed level of osmotic stress did not significantly affect the production of citric, formic, 2-keto glutaric, lactic, malic, malonic propionic and tartaric acids. Most isolates were able to solubilize tricalcium phosphate more efficiently under in vitro osmotic stress conditions compared to normal conditions. The soluble P released from tri calcium phosphate by the isolates ranged from 2.78-25.22 ppm under non osmotic conditions, while it ranged from 4.67-53.56 ppm under osmotic stress conditions. Amongst the osmotolerant bacterial cultures, Bacillus amyloliquefaciens P-72 recorded significantly higher soluble P concentrations (53.56 ppm) under osmotic stress conditions while the isolate Enterobacter sp. P-39 recorded the lowest soluble P concentration (4.98 ppm).


Keywords: Osmotolerant, Organic acids, Phosphate solubilization Rhizobacteria

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How to cite this article:

Gurupadam Hema Bindu, Govindan Selvakumar, Kaushal K. Upreti, Narayana Sunil Kumar and Duraisamy Kalaivanan. 2018. Effect of Osmotic Stress on the Organic Acid Production Profile and Tricalcium Phosphate Solubilization by Osmotolerant Bacteria.Int.J.Curr.Microbiol.App.Sci. 7(12): 1177-1183. doi: https://doi.org/10.20546/ijcmas.2018.712.146
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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