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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 1005-1012
DOI: https://doi.org/10.20546/ijcmas.2018.712.125


Effect of Nature of Explants and its Orientation on Shoot Establishment in Kufri Bahar and Kufri Surya Varieties of Potato (Solanum tuberosum L.) for Micropropagation
Deven Verma1, A.C. Yadav2 and N.R. Yadav3
1Division of Vegetable Crops, ICAR-Indian Institute of Horticultural Research, Bengaluru (560089), Karnataka, India
2Department of Vegetable Science, 3Department of Molecular Biology and Biotechnology, CCSHAU, Hisar (125004), Haryana, India
*Corresponding author
Abstract:

The experiment was conducted to study the effect of nature of explants and its orientation on shoot establishment over MS media in Kufri Bahar and Kufri Surya cultivars of potato under in vitro conditions. The results indicated that a combination of 0.4% Bavistin (25 min) + 0.1% HgCl2 (30 sec) provided the maximum number of aseptic explants (73.33%). The results have shown that in Kufri Bahar 69.18 per cent sprouts produced shoots with higher number of shoots/explant. While in case of Kufri Surya sprout’s bits responded very well (90.00%) and sprouts have produced more number of shoots/explant (6.00), average number of nodes/explants (5.12) and average shoot length (6.38 cm). The results have further shown that orienting of explants vertically, significantly increased the per cent establishment of explants (75.68% in Kufri Bahar and 70.78% in Kufri Surya), whereas, the average number of shoots, average number of nodes per explants and average shoot length was found to be more in case of horizontal orientation of explants over the media.


Keywords: Potato, Surface sterilization, Micropropagation, Sprouts, Nodal cuttings, Orientation, MS media
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How to cite this article:

Deven Verma, A.C. Yadav and Yadav, N.R. 2018. Effect of Nature of Explants and its Orientation on Shoot Establishment in Kufri Bahar and Kufri Surya Varieties of Potato (Solanum tuberosum L.) for Micropropagation.Int.J.Curr.Microbiol.App.Sci. 7(12): 1005-1012. doi: https://doi.org/10.20546/ijcmas.2018.712.125