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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 863-866
DOI: https://doi.org/10.20546/ijcmas.2018.712.107


Rapid Procedure for Genomic DNA Extraction from Pigeon Pea
Jayant Pralhad Rathod1*, Rajendra M. Gade1 and Darasing R. Rathod2
1Vasantrao Naik College of Agricultural Biotechnology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Waghapur Road, Yavatmal-445001, Maharashtra, India
2Dr. Panjabrao Deshmukh Krishi Vidyapeeth, P.O. Krishi Nagar, Akola-444104, Maharashtra, India
*Corresponding author
Abstract:

For many crucial experiments in gene cloning and further identification, purified and high quality genomic DNA is the prerequisite. In plants, polysaccharides and secondary metabolites impurities removal is complicated and time-consuming protocols are available for pigeon pea plant. In present study, rapid and proficient method for DNA extraction was optimized. The method utilizes small amount of plant material to lessen inhibitory agents like polysaccharides and phenolic compound. The method involved crushing of the young leaf in lysis buffer, incubation at 65ºC, extraction by phenol: chloroform: isoamyl alcohol and finally DNA precipitation by cold ethanol. The results showed highly pure DNA.


Keywords: DNA, Pigeon pea, Extraction, Rapid, Purity
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How to cite this article:

Jayant Pralhad Rathod, Rajendra M. Gade and Darasing R. Rathod. 2018. Rapid Procedure for Genomic DNA Extraction from Pigeon Pea.Int.J.Curr.Microbiol.App.Sci. 7(12): 863-866. doi: https://doi.org/10.20546/ijcmas.2018.712.107