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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 442-454
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.051


Prevalence, Phenotypic Characterization and Antibiotic Susceptibility of Non-Fermentative Gram Negative Bacilli Isolates at a Tertiary Care Centre
S. Prasanna1*, S. Dharanidevi1, Nikunja Kumar Das2 and Sathya Raj1
1Department of Microbiology, Shri Sathya Sai Medical College and Research Institute, Kancheepuram, Tamil Nadu, India
2DY Patil Medical college, Pune, Maharashtra, India
*Corresponding author
Abstract:

In hospitalized patients with predisposing illnesses leads to the life threatening infections with Non-fermentative Gram negative bacteria (NFGNB) like Pseudomonas aeruginosa, Acinetobacter baumannii, Stenotrophomonas maltophilia and Burkholderia cepacia. The patients are placed at high risk because the NFGNB have high intrinsic antibiotic resistance. All consecutive, non-duplicate isolates of NFGNB were collected from various clinical samples like blood, sputum, tracheal aspirate, urine, pus etc. from hospitalised patients during the study period. The phenotypic identification of various isolates was done as per standard laboratory protocol and antibiotic susceptibility interpretation was done as per CLSI guideline 2015. Out of 420 isolates the predominant sample is sputum and the predominant isolate was Pseudomonas aeruginosa followed by Acinetobacter baumannii. From the total 420 isolates, the maximum resistance was to cefepime 62 (14.8%) followed by Ceftazidime 50 (12%) and Ceftazidime-clavulanic acid 50 (12%), colistin 20 (4.8%), piperacillin-tazobactam 18 (4.3%) and gentamicin 18 (4.3%), imipenem 13 (3.1%), ciprofloxacin 12 (2.8%) and amikacin 11 (2.6%). Some of the studies from India reported the predominant isolate was Pseudomonas and some as Acinetobacter. In relation to antibiotic resistance comparatively imipenem resistance was very less than other studies that may be due to less usage of carbapenems. The main projection from our study is proper identification of NFGNBs, drug holiday and judicial usage of third and fourth generation cephalosporins and carbapenems are very important. Also implementation maintenance of strict antibiotic policy can help to fight against drug resistance by HICC.


Keywords: Non-fermentative Gram negative bacilli, Carbapenems, Pseudomonas aeruginosa, Acinetobacter baumannii.
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How to cite this article:

Prasanna, S., S. Dharanidevi, Nikunja Kumar Das and Sathya Raj. 2016. Prevalence, Phenotypic Characterization and Antibiotic Susceptibility of Non-Fermentative Gram Negative Bacilli Isolates at a Tertiary Care Centre.Int.J.Curr.Microbiol.App.Sci. 5(11): 442-454. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.051