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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 508-514
DOI: https://doi.org/10.20546/ijcmas.2018.712.063


Assessment of Genetic Fidelity of Micropropagated Gerbera (Gerbera jamesonii Bolus) Plants by ISSR Markers
R. Rashmi1*, C. Aswath2, M.V. Dhananjaya2 and D.C. Lakshmana Reddy3
1 College of Horticulture (UHS), Bagalkot, India
2Division of Floriculture and Medicinal Crops, ICAR-IIHR, Bengaluru, India
3Division of Biotechnology, ICAR-IIHR, Bengaluru, India
*Corresponding author
Abstract:

The genetic fidelity of in vitro raised gerbera plants multiplied through micropropagation upto fifteen in vitro subcultures was assessed by using inter-simple sequence repeat (ISSR) markers. 40 ISSR primers screened, 20 ISSR primers produced a total of 54 ISSRs clear, distinct and reproducible amplicons, which were monomorphic across all micropropagated plants (15) studied. Thus, a total 1094 bands were generated which exhibited homogeneous banding patterns with ISSR markers. All banding profiles from micropropagated plants were monomorphic and similar to those of the mother plant. These results indicate that the micropropagation protocol developed by us for rapid in vitro multiplication is appropriate and suitable for clonal propagation gerbera.


Keywords: Gerbera, Micropropagation, Genetic fidelity, ISSR marker
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How to cite this article:

Rashmi, R., C. Aswath, M.V. Dhananjaya and Lakshmana Reddy, D.C. 2018. Assessment of Genetic Fidelity of Micropropagated Gerbera (Gerbera jamesonii Bolus) Plants by ISSR Markers.Int.J.Curr.Microbiol.App.Sci. 7(12): 508-514. doi: https://doi.org/10.20546/ijcmas.2018.712.063