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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 447-460
DOI: https://doi.org/10.20546/ijcmas.2018.712.056


Efficacy of Native Bacillus subtilis against Postharvest Penicillium Rot Pathogen Penicillium sp. of Khasi Mandarin Oranges in Meghalaya, India
Janshame Tariang*, D. Majumder and H. Papang
Department of Plant Pathology, School of Crop Protection, College of Post Graduate Studies, Central Agricultural University, Umiam - 793103, Meghalaya, India
*Corresponding author
Abstract:

Khasi mandarin orange is an important horticulture crop in Meghalaya but several losses have been reported due to the post-harvest disease Penicillium rot caused by Penicillium sp. B. subtilis was evaluated for its efficacy against Penicillium sp. under in-vitro. A total of 260 isolates were isolated from samples collected from different habitats of Meghalaya. Twelve B. subtilis isolates were identified by morphological, biochemical and molecular identification and studied for their antagonistic properties against Penicillium sp. The habitats of these 12 B. subtilis isolates were recorded to befrom crop rhizosphere, mixed manure, fishery pond, mushroom compost and jhum area. Based on the antimicrobial traits, 6 B. subtilis isolates (Bs 167, Bs 197, Bs 217, Bs 219, Bs 256 and COB5Y1) were found with potential antagonistic properties as they were tested positive to chitinase and protease production. Bioassay showed that B. subtilis isolate COB5Y1 was recorded with the highest disease inhibition per cent against Penicillium sp. with 88.11% followed by Bs 167 (80.82%) and Bs 197 (54.82%). Four isolates COB5Y1, Bs 167, Bs 197 and Bs 217 were found with potential antagonistic properties as they could produce chitinase and protease and also were recorded with the maximum percentage of inhibition in dual test.


Keywords: B. subtilis, Penicillium rot, bio-control, Khasi mandarin, Meghalaya
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How to cite this article:

Janshame Tariang, D. Majumder and Papang, H. 2018. Efficacy of Native Bacillus subtilis against Postharvest Penicillium Rot Pathogen Penicillium sp. of Khasi Mandarin Oranges in Meghalaya, India.Int.J.Curr.Microbiol.App.Sci. 7(12): 447-460. doi: https://doi.org/10.20546/ijcmas.2018.712.056