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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 407-412
DOI: https://doi.org/10.20546/ijcmas.2018.712.051


Isolation and Molecular Identification of Probiotic Bacteria Genus Lactobacillus in an Asian Elephant (Elephas maximus) of Tamil Nadu
A. Senthilkumar1*M.G. Jayathangaraj2, A. Valli3, A. Thangavelu4,S. Gomathinayagam5 and N. Sribalaji6
1Farmers Training Centre, Theni-625 531, India
2Department of Veterinary Clinical Medicine and Therapeutics, Madras Veterinary College, Chennai-600007, India
3Institute of Animal Nutrition, Kottupakkam, Chennai-600007, India
4Department of Veterinary Microbiology, Madras Veterinary College, Chennai-600007
5Department of Veterinary Parasitology, Madras Veterinary College, Chennai-600007
6VUTRC, Salem, Tamil Nadu Veterinary and Animal Sciences University, India
*Corresponding author
Abstract:

Genus Lactobacillus was common probiotic key genera in gastro intestinal tracts of domestic animals as well as wild animals. The species compositions of probiotic bacteria are vary depending up on the animal species. The aim of this study was to isolate and molecular identification of genus Lactobacillus from dung samples of captive Asian elephants (Elephas maximus) reared in temples of Tamil Nadu state in India. Twenty five fresh dung samples of Asian elephants (Elephas maximus) were aseptically collected from various temples of Tamil Nadu state and transferred immediately to the laboratory. Isolation of Lactobacillus by culturing the dung samples using Lactobacillus MRS Broth M369 and molecular confirmation by PCR using BF1, BR1 and Lacto F, Lacto R primers. After 72 hours incubation, Lactobacillus are appeared as large whitish coloured colonies. On Gram staining, Lactobacillus organisms appeared as Gram positive long slender-rods. PCR revealed evidence of Genus Lactobacillus with amplicon sizes were 233 bp;1550 bp. Since Genus Lactobacillus are common probiotic bacteria in the gastro intestinal tract of plant eating herbivores.


Keywords: Probiotic bacteria, Genus Lactobacillus -Culturing and PCR identification- Captive Asian Elephants
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How to cite this article:

Senthilkumar, A., M.G. Jayathangaraj, A. Valli, A. Thangavelu, S. Gomathinayagam and Sribalaji, N. 2018. Isolation and Molecular Identification of Probiotic Bacteria Genus Lactobacillus in an Asian Elephant (Elephas maximus) of Tamil Nadu.Int.J.Curr.Microbiol.App.Sci. 7(12): 407-412. doi: https://doi.org/10.20546/ijcmas.2018.712.051