National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Genus Lactobacillus was common probiotic key genera in gastro intestinal tracts of domestic animals as well as wild animals. The species compositions of probiotic bacteria are vary depending up on the animal species. The aim of this study was to isolate and molecular identification of genus Lactobacillus from dung samples of captive Asian elephants (Elephas maximus) reared in temples of Tamil Nadu state in India. Twenty five fresh dung samples of Asian elephants (Elephas maximus) were aseptically collected from various temples of Tamil Nadu state and transferred immediately to the laboratory. Isolation of Lactobacillus by culturing the dung samples using Lactobacillus MRS Broth M369 and molecular confirmation by PCR using BF1, BR1 and Lacto F, Lacto R primers. After 72 hours incubation, Lactobacillus are appeared as large whitish coloured colonies. On Gram staining, Lactobacillus organisms appeared as Gram positive long slender-rods. PCR revealed evidence of Genus Lactobacillus with amplicon sizes were 233 bp;1550 bp. Since Genus Lactobacillus are common probiotic bacteria in the gastro intestinal tract of plant eating herbivores.
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