Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 242-250
DOI: https://doi.org/10.20546/ijcmas.2018.712.030


Occurrence of Escherichia coli O157:H7 in Faecal Sample of Sheep and Goats in North East Karnataka
Pralhad*, Basavaraj Awati, B.K. Ramesh, N.A. Patil, Pradeep Kumar, B. Jaganath Rao, P.T. Vinay, K.C. Mallinath, Arun Kharate, P. Suryakanth and M. Revappayya
Department of Veterinary Microbiology, Veterinary College, Bidar-585401, India
*Corresponding author
Abstract:

E. coli O157:H7 is an emerging food borne pathogen having zoonotic importance. Though the primary reservoir of the serotype is cattle; sheep and goats are also considered as main reservoir for E. coli O157:H7, which act as asymptomatic carriers. Conventional cultural and biochemical methods are time consuming to detect E. coli O157:H7 in food borne outbreaks and have less specificity and sensitivity. Latex agglutination test (LAT) tends to be specific, more rapid and reliable. In the present study, faecal samples collected from sheep (n=517) and goats (n=450) from different farms across North Eastern Karnataka during June 2016 to August 2017 were analysed. The samples were processed and analysed for the cultural isolation, biochemical characterisation and latex agglutination test. In this study, LAT was taken as confirmative diagnostic test and the results obtained by cultural isolation and biochemical characterisation were compared with that of LAT to estimate the sensitivity and the specificity. The percent of sheep and goats that showed shedding of E. coli O157 in the faeces was 3.67% (19 out of 517) and 2.88% (13 out of 450) respectively. The results obtained show that LAT is a rapid, highly sensitive, species-specific and reliable method for the detection of the pathogenic E. coli O157:H7 and could be used for identification and molecular characterisation of E. coli O157:H7 in suspected food and water borne outbreaks, disease investigations and routine analysis.


Keywords: E. coli O157: H7, sheep and goats, latex agglutination test, North East Karnataka
Download this article as Download

How to cite this article:

Pralhad, Basavaraj Awati, B.K. Ramesh, N.A. Patil, Pradeep Kumar, B. Jaganath Rao, P.T. Vinay, K.C. Mallinath, Arun Kharate, P. Suryakanth and Revappayya, M. 2018. Occurrence of Escherichia coli O157:H7 in Faecal Sample of Sheep and Goats in North East Karnataka.Int.J.Curr.Microbiol.App.Sci. 7(12): 242-250. doi: https://doi.org/10.20546/ijcmas.2018.712.030