National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
E. coli O157:H7 is an emerging food borne pathogen having zoonotic importance. Though the primary reservoir of the serotype is cattle; sheep and goats are also considered as main reservoir for E. coli O157:H7, which act as asymptomatic carriers. Conventional cultural and biochemical methods are time consuming to detect E. coli O157:H7 in food borne outbreaks and have less specificity and sensitivity. Latex agglutination test (LAT) tends to be specific, more rapid and reliable. In the present study, faecal samples collected from sheep (n=517) and goats (n=450) from different farms across North Eastern Karnataka during June 2016 to August 2017 were analysed. The samples were processed and analysed for the cultural isolation, biochemical characterisation and latex agglutination test. In this study, LAT was taken as confirmative diagnostic test and the results obtained by cultural isolation and biochemical characterisation were compared with that of LAT to estimate the sensitivity and the specificity. The percent of sheep and goats that showed shedding of E. coli O157 in the faeces was 3.67% (19 out of 517) and 2.88% (13 out of 450) respectively. The results obtained show that LAT is a rapid, highly sensitive, species-specific and reliable method for the detection of the pathogenic E. coli O157:H7 and could be used for identification and molecular characterisation of E. coli O157:H7 in suspected food and water borne outbreaks, disease investigations and routine analysis.
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