National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The current study was aimed to assess the percentage of Amp C Beta-lactamase and Extended spectrum beta-lactamase (ESBL) plus Amp C Beta-lactamase co-producer strains among urinary isolates of E. coli by disc diffusion method and to compare the evaluation of the AmpC detection by the phenotypic method using Phenyl Boronic acid and Tris-EDTA. A cross-sectional study conducted on a total of 200 non-repetitive, consecutive, urinary isolates of E. coli. The study was conducted in the Department of Microbiology of S.R.M. Medical College, Hospital and Research center, Kattangulathur. Out of the 200 isolates, a total of 47% strains were identified as the multi-drug resistant (MDR). The proportion of ESBL producing strains were 41%. Further, among the 84 clinical isolates subjected to evaluation for AmpC production, 52.38% were positive for AmpC production and 45.23% isolates were ESBL and AmpC co-producers, respectively. Of these AmpC producers(n=44), 26 (59.09%) and 18 (40.90%) isolates were identified by phenylboronic acid and TRIS-EDTA methods, respectively. Extended spectrum beta-lactamase, AmpC Beta-lactamase production, and its coexistence does exist among MDR strains of E coli. Further, this study concludes that phenylboronic acid method could be an efficient alternative for the detection of AmpC.
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