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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 388-395
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.044


Assessment of Amp C Beta-lactamase Production by Various Methods in Urinary Isolates of Escherichia coli from a Tertiary Care Teaching Hospital
Anandhi Lakshmanan1, Dharmalingam Thirunavukkarasu2*, T.Umaarasu3 and S. Rajesh2
1Department of Microbiology, ESIC Medical College and PGIMSR, Chennai, India
2Department of Microbiology, Government Mohan Kumaramangalam Medical College,Salem, Tamil Nadu, India
3Department of Microbiology, Vivekananda Dental College for Women, Tiruchengode,Namakkal, Tamil Nadu, India
*Corresponding author
Abstract:

The current study was aimed to assess the percentage of Amp C Beta-lactamase and Extended spectrum beta-lactamase (ESBL) plus Amp C Beta-lactamase co-producer strains among urinary isolates of E. coli by disc diffusion method and to compare the evaluation of the AmpC detection by the phenotypic method using Phenyl Boronic acid and Tris-EDTA. A cross-sectional study conducted on a total of 200 non-repetitive, consecutive, urinary isolates of E. coli. The study was conducted in the Department of Microbiology of S.R.M. Medical College, Hospital and Research center, Kattangulathur. Out of the 200 isolates, a total of 47% strains were identified as the multi-drug resistant (MDR). The proportion of ESBL producing strains were 41%. Further, among the 84 clinical isolates subjected to evaluation for AmpC production, 52.38% were positive for AmpC production and 45.23% isolates were ESBL and AmpC co-producers, respectively. Of these AmpC producers(n=44), 26 (59.09%) and 18 (40.90%) isolates were identified by phenylboronic acid and TRIS-EDTA methods, respectively. Extended spectrum beta-lactamase, AmpC Beta-lactamase production, and its coexistence does exist among MDR strains of E coli. Further, this study concludes that phenylboronic acid method could be an efficient alternative for the detection of AmpC.


Keywords: AmpC Beta-lactamase, Phenylboronic acid, Escherichia coli, Multidrug resistance.
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How to cite this article:

Anandhi Lakshmanan, Dharmalingam Thirunavukkarasu, T.Umaarasu and S. Rajesh. 2016. Assessment of Amp C Beta-lactamase Production by Various Methods in Urinary Isolates of Escherichia coli from a Tertiary Care Teaching HospitalInt.J.Curr.Microbiol.App.Sci. 5(11): 388-395. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.044