Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 204-215
DOI: https://doi.org/10.20546/ijcmas.2018.712.026


Diversity and Distribution of Biosurfactant Producing Plant Growth Promoting Bacteria (PGPB) from Hydrocarbon Contaminated Soils of Upper Assam
Mainu Kalita, Duarah Krondashree, Sosanka Protim Sandilya, Kamal Das, Dutta Kironta, Mahananda Chutia, Ranjana Das and Gangavarapu Subrahmanyam*
Department of Pathology, Central Muga Eri Research and Training Institute (CMER&TI), Central Silk Board, Ministry of Textiles, Govt. of India, Lahdoigarh-785700, Jorhat, Assam, India
*Corresponding author
Abstract:

Bioremediation is a highly potential approach to restore and rehabilitate oil polluted soils having many recalcitrant polyaromatic hydrocarbons (PAHs) such as dibenzothiophene (DBT), benzothiophene (BT), etc by use of microorganisms. In the present study biosurfactant producing bacterial diversity and distribution for bioremediation of oil contaminated soils of upper Assam for the growth of Muga host plant Som (Persea bombycina) were studied. Crude oil contaminated soil samples were collected from Lakwa, Gelakey, and Nazira regions of upper Assam and heterotrophic bacteria were isolated. Various screening methods for biosurfactant production as well as plant growth promoting properties were characterized for the bacterial isolates. Results of the drop collapse assay indicated the presence of biosurfactant producing bacteria. Emulsification index (EI) of 40 bacterial isolates revealed that L3, L5, L6, N2, N9, N13, N14, G1, G3, G7, G8, G9, G10, G11 isolates had higher biosurfactant production. EI values of these isolates were significantly high (> 35%) indicating potentiality higher biosurfactant production. Blue agar CTAB method was done to characterize the presence of anionic biosurfactants. Screening of PGPR activity was checked by starch hydrolysis, phosphate solubilization and extracellular enzyme screening such as catalase, lipase and cellulose. Among all the isolates, N6, N14, G1, G3, G7, G8, G9 showed good plant growth promoting characters along with biosurfactant producing ability. Heterotrophic biosurfactant producing bacterial diversity has been deciphered by 16S rRNA gene sequencing. Results indicated that abundance of Bacillus sp., Pseudomonas sp., Staphylococcus sp., Enterococcus sp., Klebsiella sp. and Serratia sp. in hydrocarbon contaminated soils.


Keywords: Bioremediation, Crude oil polluted soils, Bacterial diversity, Biosurfactant producing bacteria, Plant growth promoting bacteria
Download this article as Download

How to cite this article:

Mainu Kalita, Duarah Krondashree, Sosanka Protim Sandilya, Kamal Das, Dutta Kironta, Mahananda Chutia, Ranjana Das and Gangavarapu Subrahmanyam. 2018. Diversity and Distribution of Biosurfactant Producing Plant Growth Promoting Bacteria (PGPB) from Hydrocarbon Contaminated Soils of Upper Assam.Int.J.Curr.Microbiol.App.Sci. 7(12): 204-215. doi: https://doi.org/10.20546/ijcmas.2018.712.026