Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 141-149
DOI: https://doi.org/10.20546/ijcmas.2018.712.018


Isolation, Authentication and Evaluation of Rhizobial Isolates from the Soils of North-West Himalayas in French Bean (Phaseolus vulgaris L.)
Saroj Kumar Yadav1*, K.P. Raverkar1, Ramesh Chandra1, Navneet Pareek1 and Subhash Chandra2
1Department of Soil Science
2Departemt of Agronomy, College of Agriculture, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India
*Corresponding author
Abstract:

Nodulation ability and effectiveness of native rhizobia from the seven districts of Uttarakhand in French bean was determined. Total of 50 rhizobial isolates separated from the nodules were evaluated for their ability to form nodules and effectiveness under net house and glass house conditions. These isolates exhibited typical characteristics of Rhizobium sp. on yeast extract mannitol agar media supplemented with Congo red. Out of fifty isolates 36 were authenticated as Rhizobium based on their ability to nodulate French bean. French bean inoculation with the rhizobial isolates significantly increased the dry shoot, root and total biomass; and shoot: root ratio. The dry shoot, root and total biomass produced due to inoculation with rhizobial isolate RA6 was highest among all the rhizobial isolates. The dry shoot biomass produced due to inoculation with rhizobial isolates RA6, RL21 and RA8 was statistically at par with that of reference strain MTCC 10096. The inoculation of French bean with rhizobial isolate RA6 produced total dry biomass significantly higher by 154.4 per cent over reference strain i.e. MTCC 10096.Our results demonstrated the presence of native rhizobia in soils of N-W Himalayas capable of nodulating French bean that are either superior or at par with reference strain in improving the overall growth and synthesis of higher biomass in French bean.


Keywords: French bean, Rhizobium, Effectiveness, Nodulation, Total dry biomass
Download this article as Download

How to cite this article:

Saroj Kumar Yadav, K.P. Raverkar, Ramesh Chandra, Navneet Pareek and Subhash Chandra. 2018. Isolation, Authentication and Evaluation of Rhizobial Isolates from the Soils of North-West Himalayas in French Bean (Phaseolus vulgaris L.).Int.J.Curr.Microbiol.App.Sci. 7(12): 141-149. doi: https://doi.org/10.20546/ijcmas.2018.712.018