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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 117-124
DOI: https://doi.org/10.20546/ijcmas.2018.712.015


DNA Barcoding of Psoralea corylfolia, Mucuna pruriens and Clitoria ternatea for Species Identification
K.V. Deepak, V.A. Mohanlal*, J. Johnny Subakar Ivin and R. Anandan
Department of Genetics and Plant Breeding, Faculty of Agriculture, Annamalai University, Annamalai Nagar - 608 002, Tamil Nadu, India
*Corresponding author
Abstract:

DNA barcoding is a useful tool for species identification and phylogenetic construction. We tested the genus/species identification and phylogenetic construction in three tropical medicinal plants such as P. corylifoilia, M. pruriens and C. ternatea using a universal chloroplast DNA barcode trnH-psbA. The trnH-psbA primer pairs were used to amplify 250 bp DNA fragment in all three genera. The barcode sequences were submitted on NCBI-BLAST search and showed the nucleotide sequence similarity (homology) of 96% with P. corylifolia, 100% with M. pruriens and with 77% C. ternatea. The phylogenetic tree constructed using Neighbour joining method with the sequence of trnH-psbA revealed that grouping of the entire three different genera within the family Fabaceae.


Keywords: DNA barcode, P. corylifoilia, M. pruriens, C. ternatea, trnH-psbA, Phylogeny
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How to cite this article:

Deepak, K.V., V.A. Mohanlal, J. Johnny Subakar Ivin and Anandan, R. 2018. DNA Barcoding of Psoralea corylfolia, Mucuna pruriens and Clitoria ternatea for Species Identification.Int.J.Curr.Microbiol.App.Sci. 7(12): 117-124. doi: https://doi.org/10.20546/ijcmas.2018.712.015