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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 91-99
DOI: https://doi.org/10.20546/ijcmas.2018.712.012


Analysis of Genetic Diversity in Sclerotium rolfsii Causing Collar Rot of Chilli by RAPD
A.T. Daunde1*, K.T. Apet2 and R.L. Chavan3
1AICRP (Vegetable Crops), VNMKV, Parbhani (MS), India
2Department of Plant Pathology, VNMKV, Parbhani (MS), India
3Vilasrao Deshmukh College of Agricultural Biotechnology, VNMKV, Parbhani (MS), India
*Corresponding author
Abstract:

Collar rot of chilli caused by Sclerotium rolfsii Sacc. is currently occurring in chilli growing areas of Marathwada region in India. The genetic diversity among eight chilli S. rolfsii isolates collected from eight districts covering various geographic areas of Marathwada region of Maharashtra state were analysed using random amplified polymorphic DNA (RAPD) using five random primers. Size of DNA fragments amplified by the five primers ranged from 0.1 Kb to 10 Kb indicating polymorphism among S. rolfsii isolates. RAPD banding profiles with five different random primers viz., OPA-2, OPA-3, OPA-13, OPA-19 and OPC-5 revealed the existence of genetic variability among the isolates of S. rolfsii and were classified into two main clusters. Cluster A contains one isolate (SrA1) i.e. highly virulent. Whereas, Cluster B is sub-divided into cluster B1 (SrB2, SrO7, SrH3, SrJ4, SrL5 and SrP8) and B2 (SrN6). Hence, the present investigation confirmed the diversity among the different geographical isolates.


Keywords: Chilli, Sclerotium rolfsii, Genetic diversity, Primers, RAPD, Cluster
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How to cite this article:

Daunde, A.T., K.T. Apet and Chavan, R.L. 2018. Analysis of Genetic Diversity in Sclerotium rolfsii Causing Collar Rot of Chilli by RAPD.Int.J.Curr.Microbiol.App.Sci. 7(12): 91-99. doi: https://doi.org/10.20546/ijcmas.2018.712.012