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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2022: 95.28
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28
For more details click here
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Original Research Articles                      Volume : 7, Issue:12, December, 2018
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38
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Int.J.Curr.Microbiol.App.Sci.2018.7(12): 91-99
DOI: https://doi.org/10.20546/ijcmas.2018.712.012


Analysis of Genetic Diversity in Sclerotium rolfsii Causing Collar Rot of Chilli by RAPD
A.T. Daunde1*, K.T. Apet2 and R.L. Chavan3
1AICRP (Vegetable Crops), VNMKV, Parbhani (MS), India
2Department of Plant Pathology, VNMKV, Parbhani (MS), India
3Vilasrao Deshmukh College of Agricultural Biotechnology, VNMKV, Parbhani (MS), India
*Corresponding author
Abstract:

Collar rot of chilli caused by Sclerotium rolfsii Sacc. is currently occurring in chilli growing areas of Marathwada region in India. The genetic diversity among eight chilli S. rolfsii isolates collected from eight districts covering various geographic areas of Marathwada region of Maharashtra state were analysed using random amplified polymorphic DNA (RAPD) using five random primers. Size of DNA fragments amplified by the five primers ranged from 0.1 Kb to 10 Kb indicating polymorphism among S. rolfsii isolates. RAPD banding profiles with five different random primers viz., OPA-2, OPA-3, OPA-13, OPA-19 and OPC-5 revealed the existence of genetic variability among the isolates of S. rolfsii and were classified into two main clusters. Cluster A contains one isolate (SrA1) i.e. highly virulent. Whereas, Cluster B is sub-divided into cluster B1 (SrB2, SrO7, SrH3, SrJ4, SrL5 and SrP8) and B2 (SrN6). Hence, the present investigation confirmed the diversity among the different geographical isolates.


Keywords: Chilli, Sclerotium rolfsii, Genetic diversity, Primers, RAPD, Cluster

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How to cite this article:

Daunde, A.T., K.T. Apet and Chavan, R.L. 2018. Analysis of Genetic Diversity in Sclerotium rolfsii Causing Collar Rot of Chilli by RAPD.Int.J.Curr.Microbiol.App.Sci. 7(12): 91-99. doi: https://doi.org/10.20546/ijcmas.2018.712.012
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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