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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(12): 12-24
DOI: https://doi.org/10.20546/ijcmas.2018.712.002


Qualitative Phytochemical Screening and Gcms-Derived Fatty Acid Composition of Ethanolic Seed Extract of Cola lepidota K. Schum
O.G. Chukwuemeka1*, P.N. Okafor1, P. Nwankpa2, C.C. Etteh2, C.N. Ekweogu2, P.C. Ugwuezumba3, F.C. Emengaha2, J.N. Egwurugwu3 and D.I. Izunwanne3
1Department of Biochemistry, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria
2Department of Biochemistry, Imo State University, Owerri, Imo State, Nigeria
3Department of Medical Physiology, Imo State University, Owerri, Imo State, Nigeria
*Corresponding author
Abstract:

The qualitative phytochemical screening of the ethanolic seed extract of Cola lepidota revealed the presence of important phytochemicals. The GCMS fatty acid chromatogram showed that the extract contained fourteen fatty acid compounds and out of the fourteen compounds, five were more prominent with the peaks corresponding to the retention time range of 18.008 – 21.020. The peak at 19.779 retention time is the largest and has a peak area of 43.23%. This largest peak is identified as linoleic acid methyl ester while the second largest peak at 19.336 retention time with peak area 14.68% is due to the presence of 1, 5-cyclododecadiene. The third largest peak at 21.020 retention time with the peak area of 11.85% is Bis(2-ethylhexyl) phthalate while the fourth largest peak at 20.015 retention time with the peak area 8.98% represents octadecanoic acid methyl ester. The fifth largest peak at the retention time of 18.008 and peak area of 7.03% represents methylhexadecanoic acid. The importance of these phytochemicals is discussed.


Keywords: Cola lepidota, Retention time, Peak area, Phytochemicals, Fatty acids, Ethanolic, Seed extract
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How to cite this article:

Chukwuemeka, O.G., P.N. Okafor, P. Nwankpa, C.C. Etteh, C.N. Ekweogu, P.C. Ugwuezumba, F.C. Emengaha, J.N. Egwurugwu and Izunwanne, D.I. 2018. Qualitative Phytochemical Screening and Gcms-Derived Fatty Acid Composition of Ethanolic Seed Extract of Cola lepidota K. Schum.Int.J.Curr.Microbiol.App.Sci. 7(12): 12-24. doi: https://doi.org/10.20546/ijcmas.2018.712.002