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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 346-350
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.039


A Study on Identification and Antifungal Susceptibility Pattern of different Candida Species Isolated from Various Clinical Specimens in a Tertiary care Hospital of West Bengal, India
Indrajit Gupta1*, Purbasha Ghosh1, Ayan Nandi1, Prosenjit Naskar2, Sayantan Mondal1 and G.D. Mitra1
1Department of Microbiology, Burdwan Medical College, West Bengal, India
 2Department of Community Medicine, Burdwan Medical College, West Bengal, India
*Corresponding author
Abstract:

Cadidiasis is the 4th most common organisms causing blood-stream infection. It is mainly caused by Candida albicans but non-albicans Candida such as Candida tropicalis, Candida glabrata, Candida krusei etc has also increased in last few years. These emerging pathogenic non-albicans Candida are developing resistance to most of the conventional antifungal drugs. To find out the different Candida species in various clinical specimens and determine their antifungal susceptibility pattern. All the Candida isolates recovered from various clinical samples during the period from August 2015 to August 2016 were included in this study. The presumptive diagnosis was done on the basis of wet mount, gram’s stain, culture on Sabouraud’s dextrose agar media. Once the colonies were confirmed speciation was done by germ tube test, corn meal agar inoculation, sugar assimilation test and CHROMagar Candida inoculation. Antifungal susceptibility pattern was carried out by disc diffusion method recommended by CLSI.The antifungal agents were fluconazole (10µg), amphotericin B (10µg), ketoconazole (10 µg) and nystatin (10µg). In the present study, a total of 302 various clinical specimens were collected. Among them 126 (41.7%) Candida isolates were found. The maximum number of Candida isolates were obtained from vaginal swab (42) followed by sputum (28) and oral swab (18). The most commonly found Candida isolates were Candida albicans (52.4%) followed by C. tropicalis (27%), C. Krusei (14.3%), C. glabrata (6.3%). All the isolates of C.albicans and C.tropicalis were sensitive to amphotericin B. The next most effective antifungal drug was ketoconazole with 85.7% (108). Fluconazole was the least effective drug in our study with sensitivity of 38% (48). Candida is one of the most commonly found nosocomial pathogens. The accurate species identification of Candida is important for treatment as Non-albicans Candida are resistant to most azole group of drugs and also because of rising anti-fungal resistance among different Candida species.


Keywords: Candidiasis, Candida, non-albicans   antifungal agents.
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How to cite this article:

Indrajit Gupta, Purbasha Ghosh, Ayan Nandi, Prosenjit Naskar, Sayantan Mondal and G.D. Mitra. 2016. A Study on Identification and Antifungal Susceptibility Pattern of different Candida Species Isolated from Various Clinical Specimens in a Tertiary care Hospital of West BengalInt.J.Curr.Microbiol.App.Sci. 5(11): 346-350. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.039