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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 3423-3432
DOI: https://doi.org/10.20546/ijcmas.2018.711.393


Isolation, Enumeration and Antibiotic Sensitivity Pattern of Bacteria from the Hands of Commuters in Akure Metropolis
Ologun Oluwabusayo Mary*
Department of Microbiology, Federal University of Technology, P.M.B. 704, Akure, Nigeria
*Corresponding author
Abstract:

This study was designed to isolate and determine the antibiotic sensitivity pattern of bacteria associated with the hands of commuters in Akure metropolis. The samples were collected using sterile swab sticks at different motor parks (Obaile, Oke-Ijebu, Benin, FUTA and Road block), then transported to Microbiology laboratory for further analyses. Isolation of microorganisms was carried by direct plating on the bacteriological media. The plates were incubated at 37oC for 24 hours. Enumeration of the bacterial isolates was done by direct count of the colonies formed on the plates. Pure bacterial isolates was obtained by repeated streaking on the fresh media. Different bacteria were isolated. Staphylococcus aureus recorded high frequency (90%) while Escherichia coli recorded least frequency (12%). All the Gram negative bacterial isolates were susceptible to some of the antibiotics with varied zone of inhibition. Shigella spp was susceptible to ciprofloxacin with zone of inhibition 5.0mm. Escherichia coli and Staphylococcus aureus were resistant to pefloxacin. High microbial loads recorded from the commuters indicated the level of contamination. Therefore, hand sanitization, proper hygiene and the use of antibiotics could help in the control and spread of antibiotic resistant bacteria from fecal origin.


Keywords: Fecal bacteria, Pathogenic microorganisms, Proper hygiene, Antibiotic susceptibility, Hand-contamination
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How to cite this article:

Ologun Oluwabusayo Mary. 2018. Isolation, Enumeration and Antibiotic Sensitivity Pattern of Bacteria from the Hands of Commuters in Akure Metropolis.Int.J.Curr.Microbiol.App.Sci. 7(11): 3423-3432. doi: https://doi.org/10.20546/ijcmas.2018.711.393