Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2018) [Effective from January 1, 2018]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
journals IJCMAS
See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 3402-3408
DOI: https://doi.org/10.20546/ijcmas.2018.711.390


PCR for the Detection of Extended Spectrum β-Lactamases Genes of Pseudomonas aeruginosa
Farqad A. Aljanabi, Haider A. Alnaji, Alkarar K. Duaibel*
Department of Clinical Laboratory Investigations Techniques, Islamic University, Najaf, Iraq
*Corresponding author
Abstract:

Extended spectrum β-lactamases are often plasmid mediated and derived from mutations in bla-TEM, bla-SHV, bla-CTX-M, and bla-AmpC genes by one or more amino acid substitution around the active site. These genes are responsible for resistance to beta lactams in Pseudomonas aeruginosa. In this study, Pseudomonas aeruginosa were isolated from wound and burn infection patients admitted to Diwanyia Teaching Hospital. Routine microbiological methods were used then PCR technique for the detection of four extended spectrum β-lactamases genes (bla-TEM, bla-SHV, bla-CTX-M, and bla-AmpC). The PCR results indicated high prevalence of Extended-spectrum β-lactamases in burn infections (bla-TEM (66.7%), bla-SHV (86.7%), bla-CTX-M (93.3%), and bla-AmpC C (53.3%) and less prevalent in wound infections infected with Pseudomonas aeruginosa (bla-TEM (59.1%), bla-SHV (68.2%), bla-CTX-M(81.8%), and bla-AmpC (50%). In conclusion, Pseudomonas aeruginosa isolates of wound and burn infection patients are highly associated with emergence of extended spectrum β-lactamases genes that provide antibacterial resistance to almost broad spectrum antibiotics.


Keywords: ESBLs, Pseudomonas aeruginosa, bla-TEM, bla-SHV, bla-CTX-M, bla-AmpC, genes, PCR, MDRPA, Nosocomial infections
Download this article as Download

How to cite this article:

Farqad A. Aljanabi, Haider A. Alnaji, Alkarar K. Duaibel. 2018. PCR for the Detection of Extended Spectrum β-Lactamases Genes of Pseudomonas aeruginosa.Int.J.Curr.Microbiol.App.Sci. 7(11): 3402-3408. doi: https://doi.org/10.20546/ijcmas.2018.711.390