Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2018) [Effective from January 1, 2018]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
journals IJCMAS
See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 3201-3213
DOI: https://doi.org/10.20546/ijcmas.2018.711.368


Antimicrobial Activity of Lactobacilli against Staphylococcus aureus MRSA Strains Isolated from Mastitis in Dairy Animals
Jyotika D. Sangle, Prashant P. Mhase*, Mrunalini M. Pawade and Prerana R. Shelke
Department of Veterinary Microbiology, KNPCV, Shirwal, MAFSU, Nagpur (MS), India
*Corresponding author
Abstract:

Methicillin resistant Staphylococcus aureus (MRSA) strains being a major pathogen associated with mastitis of dairy animals, the study was proposed for investigating antimicrobial activity of lactobacilli of dairy animals having activity against the MRSA isolates of subclinical mastitis. Twenty two Lactobacilli were isolated from milk of healthy animal and screened for inherent antimicrobial activity with agar spot technique against indicator strain L. acidophilus, out of which eleven isolates exhibited strong inhibitory activity. The cell free filtrates of these isolates were then tested in vitro against MRSA by employing agar well diffusion and agar disc diffusion tests. Zone size indicative antimicrobial activity against MRSA possessing a very strong (15-18 mm) to moderately strong (10-14 mm) efficacy was observed in L. plantarum strains belonging to buffaloes (B5 and B8) and goats (G2 and G6), followed by sheep (S3). Moderate inhibitory activity (6-9 mm) was also observed for L. paraplantarum strain from cow (C2).


Keywords: Mastitis, MRSA, Lactobacilli, Antimicrobial activity
Download this article as Download

How to cite this article:

Jyotika D. Sangle, Prashant P. Mhase, Mrunalini M. Pawade and Prerana R. Shelke. 2018. Antimicrobial Activity of Lactobacilli against Staphylococcus aureus MRSA Strains Isolated from Mastitis in Dairy Animals.Int.J.Curr.Microbiol.App.Sci. 7(11): 3201-3213. doi: https://doi.org/10.20546/ijcmas.2018.711.368