National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Necrotic enteritis (NE) is sporadic disease of avian species which has been reported from most of the areas of world in which poultry are reared. The causative agent of necrotic enteritis is Clostridium perfringens, a gram-positive anaerobe. The aim of this study was isolation of C. perfringens from NE cases along with antibiotic sensitivity study of the isolates and histopathology of the NE suspected intestine. For the present study, total 50 samples were collected and inoculated in Robertson’s cooked meat media for initial enrichment followed by streaking on clostridium agar with TSC supplements and on egg yolk agar. After anaerobic incubation for 24 hr, black coloured colonies were visualized on clostridial agar (with TSC supplement) and opalescent colonies were seen on egg yolk agar. Further identification of C. perfringens was done by microscopical appearance and biochemical characterization. Total 35 (70%) samples out of 50 samples were collected and tested for the presence of C. perfringens. These isolated and pure colonies of C. perfringens were further examined for antibiotic sensitivity test. Histopathological examination of intestine of NE affected bird revealed, necrosis of villi, infiltration of heterophils, macrophages and deposition of variable number of fibrinous shreds and amorphous connective tissue which is one of the indicative findings of NE in poultry. Further study concluded RCMM as one of the best medium for enrichment of C. perfringens. Antibiotic sensitivity study of C. perfringens isolates was found that amoxicillin was the drug of choice for NE in poultry. This paper also describes the gross and microscopical important features of the intestine of NE due to C. perfringens.
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