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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 315-320
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.034


Phenotypic Detection of Carbapenemase Producing Gram Negative Bacteria by Modified Hodge Test
S.Singh*, S.A. Samant, M. Bansal, A. Talukdar and D. Arif
Department of Microbiology, MGM Medical College and Hospital, Kamothe, Navi Mumbai, India
*Corresponding author
Abstract:

The acquisition of metallo-β-lactamases (Carbapenemase) by Gram Negative Bacteria has recently emerged as one of the most worrisome resistance mechanisms, a virtue by which they can hydrolyze all beta-lactam antibiotics including penicillins, cephalosporins and carbapenems. This study was undertaken for phenotypic detection of Carbapenamase in Gram Negative Bacteria by Modified Hodge Test. A total of 200 isolates of Gram Negative Bacteria recovered from various clinical specimens. They were subjected to antimicrobial sensitivity testing and Modified Hodge Test. It was found that 46 isolates of Gram Negative Bacteria showed Carbapenamase production by Modified Hodge Test out of which 29 isolates were resistant of Carbapenems by disc diffusion. However the remaining 17 isolates could be potential Carbapenamase producers and may lead to treatment failure. Hence it is necessary to carry out Modified Hodge Test for Gram Negative Bacteria.


Keywords: Metallo-β-lactamases,Modified Hodge Test,beta-lactam antibiotics
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How to cite this article:

Singh, S., S.A. Samant, M. Bansal, A. Talukdar and Arif, D. 2016. Phenotypic Detection of Carbapenemase Producing Gram Negative Bacteria by Modified Hodge TestInt.J.Curr.Microbiol.App.Sci. 5(11): 315-320. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.034