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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 3036-3041
DOI: https://doi.org/10.20546/ijcmas.2018.711.348


RP-HPLC Method for Determination of Organophosphate and Synthetic Pyrethroids Insecticides
Romila Akoijam1*, Arati Ningombam1, Aruna Beemrote1 and R.S. Telem2
1ICAR Research Complex for North Eastern Hill Region, Manipur Centre, Lamphelpat, India
2Farm Science Centre (KVK), Hengbung, Senapati, India
*Corresponding author
Abstract:

A modified QuEChERS method for simultaneous detection of monocrotophos, deltamethrin, phosphamidon and dichlorovos insecticides in cabbage and cauliflower samples was developed. The detection was accomplished with the use of reverse phase high performance chromatograph (RP-HPLC) coupled with C18 column with UV-VIS detection system. The method employs the primary secondary amine (PSA) sorbent and activated charcoals were used for extraction and clean-up process. The quantification of monocrotophos and deltamethrin was done with an isocratic flow of 1.70 mL min-1 of mobile phase of acetonitrile and water (90:10, v/v) at 220 nm wavelength. The insecticides phosphamidon and dichlorovos was separated with an isocratic flow of 1.50 mL min-1 acetonitrile and water (70:30, v/v) at 210 nm wavelength. The peaks of monocrotophos and deltamethrin were depicted at retention times of 0.76 min and 2.85 min and the peaks of phosphamidon and dichlorovos was found at 1.10 min and 1.37 mins. The recovery percentage found at different levels of 0.05, 0.10, 0.25, 0.50 and 1.00 mg kg-1 levels were above 80 % for all the insecticides.


Keywords: Monocrotophos, Deltamethrin, Cabbage, Cauliflower, HPLC
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How to cite this article:

Romila Akoijam, Arati Ningombam, Aruna Beemrote and Telem, R.S. 2018. RP-HPLC Method for Determination of Organophosphate and Synthetic Pyrethroids Insecticides.Int.J.Curr.Microbiol.App.Sci. 7(11): 3036-3041. doi: https://doi.org/10.20546/ijcmas.2018.711.348