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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 3030-3035
DOI: https://doi.org/10.20546/ijcmas.2018.711.347


Development of Modified QuEChERS Methodology Coupled with RP-HPLC for Quantification of Various Insecticides in Rice Grains
Romila Akoijam1*, Arati Ningombam1, Aruna Beemrote1 and R.S. Telem2
1ICAR Research Complex for North Eastern Hill region, Manipur Centre, Lamphelpat, India
2KVK, Hengbung, Senapati, India
*Corresponding author
Abstract:

A method of reverse phase-high performance liquid chromatography (HPLC) was standardized and developed coupled with modified UV-VIS detection system was standardized for simultaneous determination of residues of imidacloprid, carbofuran, chlorantraniliprole, fipronil, malathion, chloropyriphos and cypermethrin in rice grains. Extraction and clean-up process was done by modified QuEChERS method. The Brownlee Analytical C18 column was used for separation with an isocratic flow @ 0.5 mL min-1 of mobile phase of acetonitrile and water (90:10, v/v) was operated. The peaks of imidacloprid, carbofuran, chlorantraniliprole, fipronil, malathion, chloropyriphos and Cypermethrin A, B and C in chromatograms depicted at retention times of 2.67 min, 3.25 min, 3.54 min, 3.93 min, 4.08 min, 8.19 min and 9.32, 9.78 and 10.00 mins respectively. Constant recoveries were observed above 80 % for all the three insecticides when samples were fortified at different levels of 0.05, 0.10, 0.25, 0.50 and 1.00 mg kg-1 levels.


Keywords: Imidacloprid, Carbofuran, Rice grains, HPLC
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How to cite this article:

Romila Akoijam, Arati Ningombam, Aruna Beemrote and Telem, R.S. 2018. Development of Modified QuEChERS Methodology Coupled with RP-HPLC for Quantification of Various Insecticides in Rice Grains.Int.J.Curr.Microbiol.App.Sci. 7(11): 3030-3035. doi: https://doi.org/10.20546/ijcmas.2018.711.347