National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A method of reverse phase-high performance liquid chromatography (HPLC) was standardized and developed coupled with modified UV-VIS detection system was standardized for simultaneous determination of residues of imidacloprid, carbofuran, chlorantraniliprole, fipronil, malathion, chloropyriphos and cypermethrin in rice grains. Extraction and clean-up process was done by modified QuEChERS method. The Brownlee Analytical C18 column was used for separation with an isocratic flow @ 0.5 mL min-1 of mobile phase of acetonitrile and water (90:10, v/v) was operated. The peaks of imidacloprid, carbofuran, chlorantraniliprole, fipronil, malathion, chloropyriphos and Cypermethrin A, B and C in chromatograms depicted at retention times of 2.67 min, 3.25 min, 3.54 min, 3.93 min, 4.08 min, 8.19 min and 9.32, 9.78 and 10.00 mins respectively. Constant recoveries were observed above 80 % for all the three insecticides when samples were fortified at different levels of 0.05, 0.10, 0.25, 0.50 and 1.00 mg kg-1 levels.
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