National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Caralluma quadrangula (Forssk) N.E. Br belongs to the Asclepiadaceae family and was collected from the Al-Shafa Mountain in Taif, a city in the Mecca Province of Saudi Arabia at an elevation of 1,879 m (6,165 ft) on the slopes of the Sarawat Mountains. The plant’s latex was extracted and dissolved into five different aqueous solutions with the solvents: distilled water, Zamzam water, acetone, ethyl alcohol and Tris-HCl to yield five different extracts. The objectives of this research is to extract Caralluma quadrangula latex using different aqueous solutions / solvents and to evaluate the inhibition zones using 5 pathogenic and non pathogenic microorganisms using six various concentrations of Caralluma quadrangula latex. This study on Caralluma quadrangula (Forssk) N.E. Br, obtained from Al- Shafa, Taif. The research was conducted at the Department of Biotechnology, Taif University. The bacterial strains from ATCC and the nutrient Hiveg- Agar from Himedia were utilized for the study. The antimicrobial activity was tested against both Fungi, Gram-positive and Gram-negative bacteria using the agar diffusion method. Antimicrobial activities were observed, especially against Micrococcus luteus, Candida albicans, Escherichia coli, and Pseudomonas aeruginosa strains. The higher activity was exhibited in the acetonic and ethyl alcohol extract solutions of Caralluma quadrangula latex depending upon various concentrations towards the particular organism. These results are encouraging due to the fact that the plants are rich in antimicrobial sources that should be considered; especially antimicrobial peptides from latex extracted in Zamzam water and compounds were extracted from latex in solvent, further studies should be carried out to confirm the purification of the antimicrobial compounds.
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