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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 269-274
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.029


Metallo Beta Lactamase Producing Pseudomonas aeruginosa in a Tertiary Care Hospital
P.R. Lyra1*, Sindhu Cugati2, K. Anuradha3 and D. Venkatesha4
1Department of Microbiology, ACME, Pariyaram, India
2Department of Microbiology, Sree Balaji Medical College and Hospital, Chennai, India
3Department of Microbiology, Mysore Medical College and Research Institute, Mysore, India
4Department of Microbiology, Adichunchanagiri Institute of Medical Science, Bellur, India
*Corresponding author
Abstract:

Acquired drug resistance is frequent in nosocomial isolates of Pseudomonas aeruginosa. Acquired metallo-beta-lactamases (MBL) have recently emerged as one of the most worrisome resistance mechanism. The occurrence of an MBL positive isolate in a hospital environment poses not only a therapeutic problem, but is also a serious concern for infection control management. Hence the present study was conducted to detect the prevalence of MBL producing P.aeruginosa in a tertiary care hospital and to formulate antibiotic policy and plan a proper hospital infection control strategy to prevent the spread of these strains. A total of 100 Pseudomonas aeruginosa isolated from various clinical specimens over a period of one year from January 2011 to December 2011 in the Department of Microbiology, Mysore Medical College and Research Institute, Mysore were selected for the study. These isolates were subjected to antibiotic susceptibility testing and screened for MBL production using Imipenem- EDTA combined disc test. Out of the 100 Pseudomonas aeruginosa isolated, 22 (22%) were MBL producers. 36.4% were from pus, followed by blood (22.7%), urine (18.2%), CSF (13.6%), sputum and endotracheal aspirate (4.5%) respectively.100(100%) of the isolates were resistant to Ceftazidime, Cefotaxime, Cefepime and Piperacillin.16 (72.7%) were sensitive to Aztreonam, and 10(45.5%) to Imipinem.  Multidrug resistance is common in P.aeruginosa. This study highlights the need to do surveillance to detect MBL producers, judicious use of antibiotics and implementation of appropriate infection control measures to control the spread of these strains in the hospital.


Keywords: Pseudomonas aeruginosa, Kirby-Bauer disc diffusion technique, Metallo-beta- lactamase, Combined disc test, Multidrug Resistance, Imipenem.
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How to cite this article:

Lyra, P.R., Sindhu Cugati, K. Anuradha and Venkatesha, D. 2016. Metallo Beta Lactamase Producing Pseudomonas aeruginosa in a Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 5(11): 269-274. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.029