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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 2645-2652
DOI: https://doi.org/10.20546/ijcmas.2018.711.302


Influence of Growth Hormones on Initiation of Somatic Embryogenesis in Coconut var. Chowghat Orange Dwarf
R. Renuka*, J. Ann Greeshma, N. Nirmala and R. Meera
Plant Tissue Culture Laboratory, Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore – 641 003, India
*Corresponding author
Abstract:

Coconut (Cocos nucifera L.), is an important perennial crop cultivated across the globe. The demand for quality planting materials is increasing across the world for the establishment of new orchards, rejuvenation of existing orchards and production of quality nuts. Plant tissue culture is an alternate approach for the production of quality planting material. In this investigation, influence of auxin and cytokinin on somatic embryogenesis in coconut is studied using sliced mature embryos as explants in modified Y3 media supplemented with various concentrations of 2,4-D and kinetin. Maximum embryogenic calli and translucent structures were formed in Y3 modified media supplemented with equal concentration of 2,4-D and kinetin (100 µM, 200 µM and 300 µM). Maximum embryogenic structures were formed in Y3 media supplemented with high concentration of 2,4-D (450 µM) and with lower concentration of Kinetin (100 µM). Further, these embryogenic structures on culturing in media supplemented with cytokinin will form globular embryos.


Keywords: Coconut, Embryogenic calli, Translucent structures, Embryogenic structures, 2,4-D and kinetin
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How to cite this article:

Renuka, R., J. Ann Greeshma, N. Nirmala and Meera, R. 2018. Influence of Growth Hormones on Initiation of Somatic Embryogenesis in Coconut var. Chowghat Orange Dwarf.Int.J.Curr.Microbiol.App.Sci. 7(11): 2645-2652. doi: https://doi.org/10.20546/ijcmas.2018.711.302