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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 2500-2506
DOI: https://doi.org/10.20546/ijcmas.2018.711.284


Studies on Morphological, Cultural and Pathogenic Variability in Isolates of Fusarium oxysporum f.sp. pisi causing Wilt of Pea from Different Districts of Manipur
N. Kripalini1, Mohan Kumar Biswas1 and Ph. Sobita Devi2
1Department of Plant Protection, Palli- Siksha Bhavana, Sriniketan West Bengal-731236, India
2Department of Plant Pathology, College of Agriculture, CAU, Iroisemba, Imphal-795005, India
*Corresponding author
Abstract:

Pea is one of the most important rabi pulse crops of India. Pea is being rich in protein are valuable for vegetable purpose. Fourteen isolates of Fusarium oxysporum f.sp.pisi from different districts of Manipur were studied for its cultural, morphological and pathogenic variability. The mycelia colour varied from white to light pink, purple and pale yellow colour. The radial growth of the isolates ranged from 5.4cm to 8.9cm at 8 days after inoculation at 26±10 0C in 90mm petriplates. Sporulation of all isolates showed moderate to profuse. The size of micro conidia ranged from 11.6x 3.1 to 25.2 x 6.2 µm and size of micro conidia ranged from 3.02x 2.1 µm to 9.2 x 5.6 µm. The number of septation of macroconidia was mostly 2-3 whereas in microconidia most of the isolates was found with no septum. The shape of the macroconidia is mostly sickle shaped. In microconidia most of the isolates are oval shaped. The conidial colours of all the isolates were found hyaline. The formation of chlamydospores ranged from ten to twentyone numbers of all the isolates on Potato Dextrose Agar medium. The dry weight mycelium showed variability in the weight of isolates which was ranged from 0.78 to 1.31 gm. In pathogenic variability, all the isolates exhibited wilt symptom and hence proved pathogenic on pea cultivars under artificial inoculation.


Keywords: Pea, Fusarium oxysporum f.sp.pisi, Cultural, Morphological, Pathological and variability
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How to cite this article:

Kripalini, N., Mohan Kumar Biswas and Ph. Sobita Devi. 2018. Studies on Morphological, Cultural and Pathogenic Variability in Isolates of Fusarium oxysporum f.sp. pisi Causing Wilt of Pea from Different Districts of ManipurInt.J.Curr.Microbiol.App.Sci. 7(11): 2500-2506. doi: https://doi.org/10.20546/ijcmas.2018.711.284