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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 2461-2472
DOI: https://doi.org/10.20546/ijcmas.2018.711.281


A Study on the Incidence of Shiga Toxin Producing Escherichia coli in Dairy Cattle and Its Environment
K. Venkateswara Rao1, A. Jagadeesh Babu2, T. Madhava Rao2, Chinta Siva Swetha2*, P. Ramya4, Deepak3 and S. Somasekhar1
1Department of Animal Husbandry, Andhra Pradesh, India
2Department of Veterinary Public Health and Epidemiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati- 517502, India
3Department of Animal Husbandry, Karnataka, India
*Corresponding author
Abstract:

The present study was designed to study the prevalence and characterization of shiga toxin producing Escherichia coli from dairy animals, water samples in dairy farms and from humans who were in close contact with animals. A total of 508 faecal samples from dairy animals, 4 water samples from dairy farms and 28 Human faecal samples, who were in close contact with animals were subjected to cultural and biochemical tests for confirmation of E. coli. The molecular characterization of positive isolates with stx1, stx2, eaeA and hlyA genes and multiplex PCR for confirmation. A total 522 samples were positive for E. coli out of 540 (96.67%) samples by culture method, 319 (61.11%) positive on blood agar plate assay and various biochemical characteristics were studied. Out of 522 positive E. coli, 56 (10.72%) are positive for STEC by PCR method. On multiplex PCR of stx1, stx2, eaeA and hlyA genes 6 were positive 6 out of 56 (8.92%). The frequent isolation of STEC strains from non human sources like animals, food and other products along with the identification of multidrug resistance and virulence genes across the Indian subcontinent poses a serious threat of the outbreaks in humans that can occur in the future.


Keywords: Escherichia coli, Shiga toxin, PCR, Faecal samples and water samples
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How to cite this article:

Venkateswara Rao, K., A. Jagadeesh Babu, T. Madhava Rao, Chinta Siva Swetha, P. Ramya, Deepak and Somasekhar, S. 2018. A Study on the Incidence of Shiga Toxin Producing Escherichia coli in Dairy Cattle and Its Environment.Int.J.Curr.Microbiol.App.Sci. 7(11): 2461-2472. doi: https://doi.org/10.20546/ijcmas.2018.711.281