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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 240-256
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.026


Antimicrobial Activities Evaluation from the Extracts of Leaves, Flowers, Fruits and Latex of Calotropis procera from Taif
Abd-El Aziem Farouk1,2*, N. Thoufeek Ahamed1, Othman AlZahrani1, Khalid H. Alamer3 and Abdul Aziz Bahobail3
1Molecular Biotechnology Research Unit, Department of Biotechnology, Faculty of Science, Taif University, Al-Hawiya 888, Kingdom of Saudi Arabia
2Tsuno GmbH, Wibiloweg 16, 69123 Heidelberg, Germany
3Department of Biology, Faculty of Science, Taif University, Al-Hawiya 888,Kingdom   of Saudi Arabia
*Corresponding author
Abstract:

Antimicrobial activities evaluated from leaves, flowers, fruits and latex of locally obtained Calotropis procera from Al-Sharifieya, Taif in different aqueous solutions and solvents such as sterilized Zamzam water and distilled water, ethanol, acetone, isoamyl alcohol and 100 mM Tris-HCl at pH 8.7 by using liquid Nitrogen. Calotropis procera was subjected to a standard agar well diffusion method. The ethyl alcohol extract of leaves of Calotropis procera showed an activity against both Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATCC 10231 with a concentration of 200 mg/ml clearing zones 10 mm in diameter. The Tris-HCl at pH 8.9 (100 mM) extracts of leaves extracts also showed the highest antimicrobial activity against Pseudomonas aeruginosa ATCC 27853 with same concentrations (diameter 12 mm).  The ethyl alcohol extracts of flower and latex showed antimicrobial activity against Bacillus subtilis ATCC 6633 with same concentrations (diameter 10 mm and 8 mm). Among the different solvent extracts of Calotropis procera, the distilled water and Zamzam water extracts of leaves, flower, fruit, latex and Tris-HCl pH 8.9 (100 mM) extracts of fruits and latex showed activity against Bacillus subtilis ATCC6633 with the same concentration (diameter 8 mm). Similar inhibitions (zones of 4-6 mm) for Zamzam and sterilized distilled water extracts of fruit of Calotropis procera were seen for the Escherichia coli ATCC 8739, Micrococcus luteus ATCC 9341 and Pseudomonas aeruginosa ATCC 27853. In isoamyl alcohol and acetone extracts of fruit activity was observed in all four bacteria


Keywords: Agar well diffusion method, antimicrobial activities, Calotropis procera, medicinal plant extract.
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How to cite this article:

Abd-El Aziem Farouk, N. Thoufeek Ahamed, Othman AlZahrani, Khalid H. Alamer and Abdul Aziz Bahobail. 2016. Antimicrobial Activities Evaluation from the Extracts of Leaves, Flowers, Fruits and Latex of Calotropis procera from Taif.Int.J.Curr.Microbiol.App.Sci. 5(11): 240-256. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.026