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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 222-229
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.024


Validity of Different Laboratory Methods for Diagnosis of Infectious Vaginitis: Real-Time Polymerase Chain Reaction for Detection of Trichomoniasis
Suzan F. Ali1, Walaa Othman Elshabrawy2*, Muneera A. Alsheeha3, and Ahmed Hussein4
1Departments of Medical Microbiology
2Clinical Pathology, 3Obstetrics and Gynecology and 4Center of Medical Research, College of Medicine, Qassim University, KSA
*Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Egypt
*Corresponding author
Abstract:

Vaginitis syndrome is one of the most common reasons for women to seek medical attention that is mostly infectious. The aim of this study was to validate the different laboratory methods for diagnosis of infectious vaginitis and to implement the role of molecular method for detection of T. vaginalis in comparison to routine diagnostic techniques. Four vaginal swabs were collected from each woman and subjected to full microbiological laboratory studies involving direct microscopic examinations and isolation techniques. In addition, A 145 bp long fragment of the repeated DNA target from the genome of Trichomonas vaginalis is amplified with specific primers and detected with hybridization probes using real-time PCR. All specimens were negative for Trichomonas vaginalis in wet mount preparations and twenty eight percent of cases were positive for fungal elements and bacterial agents in Gram stained smears. Forty one cases yielded positive culture, sixteen bacterial isolates, thirteen fungal isolates and no isolates of T.vaginalis were detected. Mixed bacterial and Candida isolates were found in twelve cases. Nine specimens tested positive using real-time PCR resulting in 9.4% prevalence of trichomoniasis in the women studied. From this study we can conclude that the direct microscopic examination and culture methods used shows reasonable sensitivity for detection of BV and vaginal candidiasis but not for T. vaginalis among the studied cases. Candidal and bacterial vaginosis can coexist. The real-time PCR was found more sensitive and rapid method for detection of T. vaginalis.


Keywords: Bacterial,candidal and trichomonal vaginitis;wet mount;Real-time PCR.
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How to cite this article:

Suzan F. Ali, Walaa Othman Elshabrawy, Muneera A. Alsheeha and Ahmed Hussein. 2016. Validity of Different Laboratory Methods for Diagnosis of Infectious Vaginitis: Real-Time Polymerase Chain Reaction for Detection of Trichomoniasis.Int.J.Curr.Microbiol.App.Sci. 5(11): 222-229. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.024