Medicinal plants, plays vital role in curing diseases due to their antimicrobial and antifungal activity against human pathogens through decades. Herbal Mouthwashes are in high demand, because thy act on oral pathogens and relieve the pain instantly and are also less side effective. One of the most common infectious diseases encountered by many individuals are Dental carries and Periodontal diseases at different stages of their life time. Dental caries include the cavity formation, eruption of enamel, swollen gums, bleeding gums, formation of hollow black eruption on the surface of the teeth. In early days, Dental caries are high among Children and Adolescents, because they do not practice proper oral hygiene. To prepare Anti-bacterial Herbal Mouthwash from the aqueous extracts of 4 different leaves namely Azadirachta indica (Neem), Ocimum basilicum (Tulsi), Mentha longifolia (Mint), Punica granetum Linn (Pomegranate) and rhizomes of Curcuma longa (Turmeric) that acts against the oral pathogens- Staphylococcus aureus, Streptococcus pyogenes, Lactobacillus bulgaricus, Bacillus subtilis and Escherchia coli and to check the Anti-microbial activity by using Agar well diffusion method. Punica granetum Linn(Pomegranate) shows sensitivity towards the Streptococcus pyogenes, Lactobacillus bulgaricus, Staphylococcus aureus, Bacillus subtilis and Escherchia coli. Azadirachta indica (Neem) shows sensitivity towards Staphylococcus aureus and Escherchia coli and shows resistance towards Streptococcus pyogenes, Lactobacillus bulgaricus and Bacillus subtilis. Ocimum baslicum (Tulsi) shows sensitivity towards Staphylococcus aureus, Bacillus subtilis and Escherchia coli and shows resistance towards Streptococcus pyogenes, Lactobacillus bulgaricus. Mentha longifolia (Mint) shows sensitivity towards Staphylococcus aureus, Lactobacillus bulgaricus and Escherchia coli and shows resistance towards Streptococcus pyogenes and Bacillus subtilis. Curcuma longa (Turmeric) shows sensitivity towards Lactobacillus bulgaricus, Staphylococcus aureus and Escherchia coli and shows resistance towards Streptococcus pyogenes, Bacillus subtilis. Hence this study proves that Punica granetum Linn(Pomegranate) shows effectiveness against all the 5 oral bacterias namely Streptococcus pyogenes, Lactobacillus bulgaricus, Staphylococcus aureus, Bacillus subtilis and Escherchia coli. Pomegranate leaves possess flavonoids which is used as an anti-oxidant in conditions of Xerotomia. Thereby, Pomegranate leaves can also be included in the preparation of Herbal mouthwashes.

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