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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 2153-2162
DOI: https://doi.org/10.20546/ijcmas.2018.711.242


Serological, Microscopical and Molecular Examinations of Cow Milk Samples for Diagnosis of Paratuberculosis at Mhow of Madhya Pradesh in India
Matoli1, S.D. Audarya1*, D. Chhabra1, M. Singh2, K. Chaubey2, S. Gupta2, Sikrodia1, G.P. Jatav3 and S.V. Singh2
1Department of Veterinary Microbiology
3Department of Veterinary Pathology,
 College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Mhow-453446, Indore, Madhya Pradesh, India
2Veterinary Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India
*Corresponding author
Abstract:

Mycobacterium avium subspecies paratuberculosis (MAP) is a Gram positive, acid-fast bacterium which causes Johne’s disease or Paratuberculosis in domestic livestock species. For screening of cows for MAP infection a total of 103 milk samples from individual cows were collected from organized (57) and unorganized (46) farms. Presence of anti-MAP antibodies in milk were screened by indirect enzyme linked immunosorbent assays (iELISA) test, 72 (69.90%) cow milk samples were found positives (66.07% from organized farms and 74.46% from unorganized farms). Positivity percentages were 62.50 in Gir cows and 70.52 in non-descriptive and crossbred cows irrespective of the farming system for the presence of MAP antibodies. Milk samples from 13 clinically suspected cows were used to prepare smears on glass slides and stained by Ziehl-Neelsen method. Microscopically 2 cow milk samples were found positive for presence of acid-fast organisms. Extracted nucleic acid, deoxyribose nucleic acid (DNA) from strong positive milk sample in microscopy was further tested in polymerase chain reaction (PCR) to amplify MAP DNA using insertion sequence 900 (IS900) specific primers. Except in positive control, specific amplifications were not visualized for test samples after agar gel electrophoresis. The present study indicates circulation of Mycobacterium avium subspecies paratuberculosis in cattle population of Mhow of Indore in Madhya Pradesh.


Keywords: Acid-fast bacilli, Cow, iELISA, Paratuberculosis
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How to cite this article:

Matoli S., S. D. Audarya, D. Chhabra, M. Singh, K. Chaubey, S. Gupta, R. Sikrodia, G. P. Jatav and Singh S. V. 2018. Serological, Microscopical and Molecular Examinations of Cow Milk Samples for Diagnosis of Paratuberculosis at Mhow of Madhya Pradesh in India.Int.J.Curr.Microbiol.App.Sci. 7(11): 2153-2162. doi: https://doi.org/10.20546/ijcmas.2018.711.242