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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 2020-2026
DOI: https://doi.org/10.20546/ijcmas.2018.711.229


Isolation and Characterisation of Bioactive Peptides Components from Caprine Milk Casein Fractions
H. Devaraja Naik1*, M. Venkatesh2 and H. Arun Kumar2
1Department of Livestock products Technology, Veterinary College, Karnataka Veterinary, Animal and Fisheries Science University (Bidar), Hassan, India
2Department of Dairy Technology, Dairy Science College, Karnataka Veterinary, Animal and Fisheries Science University (Bidar), Hebbal Bengaluru, India
*Corresponding author
Abstract:

The caprine milk proteins are considered as power house of bioactive peptides, these bioactive peptides may be released in enzymatic hydrolysis(Trypsin 10 mg of enzyme/5 g of protein) at pH 8.0 and temperature 400 C was of casein fractions was carried out at an enzyme- substrate (E: S) ratio of 1:25. Enzymatic hydrolysis of casein fractions was carried out at an enzyme- substrate (E: S) ratio of 1:25. The milk protein from goat milk was extracted by using acid precipitation method and purified using urea fractionation. Fractionation of whole casein from 6.6 M urea to 4.63M urea yields a precipitate of αs-casein and β- casein is obtained by further dilution of the supernatant to 1.7 M urea at pH of 4.7. Enzymatic hydrolysis of casein fractions was carried out at an enzyme- substrate (E: S) ratio of 1:25 The SDS-PAGE was most widely used method for analyzing protein mixtures qualitatively and RP-HPLC was useful for monitoring protein purification of Bioactive peptides (BAPs). Hydrolysed bioactive peptides of caprine milk proteins are exhibit various physiological effects in the body, such as gastrointestinal, cardiovascular, endocrine, immune, and nervous systems.


Keywords: Caprine milk, Fractionation, Characterisation, Casein hydrolysates, Bioactive peptides, SDS-PAGE, RP-HPLC
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How to cite this article:

Devaraja Naik, H., M. Venkatesh and Arun Kumar, H. 2018. Isolation and Characterisation of Bioactive Peptides Components from Caprine Milk Casein Fractions.Int.J.Curr.Microbiol.App.Sci. 7(11): 2020-2026. doi: https://doi.org/10.20546/ijcmas.2018.711.229