National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Total of (104) urine samples were collected from patients suffering from urinary tract infection with different age groups from five hospitals in Baghdad (Ibn- Albalady, Al Yarmouk, Medical city, Baghdad hospital and Al-Kandy) from the period of the beginning of September 2015 to the end of December 2015.All samples were examined by traditional methods based on cultural characteristics, biochemical test and API 20 strep. The results showed the revealed of 50 isolates to Enterococcus and this confirmed by polymerase chain reaction technique based on amplification of species specific genes. Antimicrobial susceptibility testing of the fifty isolates were screened by disc diffusion method on Mueller-Hinton agar using 10 types of antibiotics which differ in their action includes (Ampicillin, Cefepime, Imipenem, Vancomycin, Rifampicin, Erythromycin, Ofloxacin, Oxacillin Oxytetracycline, and Streptomycin),results show 100% of isolates resist to six types of antibiotics (Cefepime, vancomycin, rifampicin, erythromycin, oxacillin and oxytetracyclin) and showed different pattern of resistance to the others four antibiotics. On the other hand we use two methods, Congo red agar(CRA) and microtitre plate method (MTP) to detect Enterococcus biofilm production. Results of (CRA) methods show that 22(44%) detect as strong, 25(50%) as moderate and 3(6%) as week biofilm production, while for (MTP) method results show that 20(40%) detect as strong, 26(52%) as moderate and 4(8%) as week biofilm production. This study aimed to describe antibiotic resistance of E. faecalis and E. faecium and it role in biofilm formation from bacterial isolates.
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