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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 1700-1707
DOI: https://doi.org/10.20546/ijcmas.2018.711.194


Cloning, Sequencing and Phylogenetic Analysis of inv E Gene of Salmonella typhimurium
Yashpal Singh1, Apoorv Tiwari1, Rajesh Kumar2 and M.K. Saxena3*
1Department of Molecular Biology and Genetic Engineering
2Department of Veterinary Microbiology
3Department of Veterinary Physiology & Biochemistry, G.B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India
*Corresponding author
Abstract:

Salmonella enterica subsp. enterica serovar typhimurium is a gram-negative serious contagious pathogen having a broad host range. It has been associated with a wide spectrum of infectious diseases, including typhoid fever and nontyphoidal salmonellosis, which cause public health problems worldwide. Several genes contribute in virulence and determine pathogenicity of this isolate. inv E is an important invasion gene, member of Salmonella pathogenicity island1 (SPI-1) which helps pathogen to invade the host intestinal epithelial cell and also mediates the translocation of several bacterial effector proteins that have the capacity to stimulate cellular responses leading to bacterial internalization and the reprogramming of gene expression in the infected cells. In present study inv E gene was cloned, sequenced and phylogenetic analysis was done using bioinformatics approach. Genomic DNA was isolated from field isolate of Salmonella Typhimurium (A-201) and inv E gene was amplified using gene specific primers and cloned in pJET vector by the positive selection system. Amplification of inv E gene yielded a product of approximately 1.1 kb. Subsequently gene was sequenced and a complete ORF of 1119 bp was obtained. The sequence was submitted to NCBI Genbank and Accession No. KC607873 was allocated. The sequence was analysed using bioinformatics tools for the homologous sequences. Sequence homology was searched at nucleotide level and exhibited 99% similarity with Salmonella enterica strain FORC030 complete genome and Salmonella enterica subsp. enterica serovar Indiana strain D90, complete genome.


Keywords: Salmonella, Gene, Protein, Vaccine
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How to cite this article:

Yashpal Singh, Apoorv Tiwari, Rajesh Kumar and Saxena, M.K. 2018. Cloning, Sequencing and Phylogenetic Analysis of inv E Gene of Salmonella typhimurium.Int.J.Curr.Microbiol.App.Sci. 7(11): 1700-1707. doi: https://doi.org/10.20546/ijcmas.2018.711.194