National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Molecular detection of Banana Bunchy Top Virus (BBTV) was carried out using nucleic acid based polymerase chain reaction (PCR) method with six different primer pairs for the six components of BBTV (DNA1 to DNA6) genome. Samples were collected from six banana cultivars exhibiting the characteristic symptoms Banana bunchy top disease from eight districts of Assam viz., Jorhat, Golaghat, Sonitpur, Morigaon, Nagaon, Kamrup Metro, Kamrup Rural and Goalpara. All the primer pairs detected the BBTV positive samples giving a band size of 1111 bp for DNA 1, 1058 bp for DNA 2, 1075 bp for DNA 3, 1046 bp for DNA 4, 1018 bp for DNA 5 and 1089 bp for DNA 6. Results revealed the prevalence of BBTV in all the surveyed banana orchards. Twelve PCR products were partially sequenced and compared with those of BBTV reported previously. The sequence similarity and phylogenetic analysis showed 86.00 to 99.00 per cent similarity with known isolates of Pacific Indian Ocean (PIO) group members of BBTV. However, two isolates showed 94 to 95 per cent similarity with members of South-East Asian (SEA) group of BBTV. Present investigations showed an indication of prevalence of genetic distinctiveness of BBTV Assam isolates with known Indian isolates.
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