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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 792-814
DOI: https://doi.org/10.20546/ijcmas.2018.711.095


Genomic Analysis of Rotavirus Species A Isolated from Swine, Amazon Region, Brazil
Mayara A.O. Neves1, Daniel S. De Camargo2, Kathleen V.S. de Araújo³, Patrícia S. Lobo³, Renato Da S. Bandeira¹, Luana S. Soares³ and Joana D.P. Mascarenhas³*
1Postgraduate Program in Virology, Evandro Chagas Institute, Ananindeua, Pará, Brazil
2Center for Tropical Medicine, Federal University of Pará, Pará, Brazil
3Virology Section, Evandro Chagas Institute, Health Surveillance Secretariat, Brazilian Ministry of Health, Ananindeua, Pará, Brazil
*Corresponding author
Abstract:

This present study has aimed to provide a molecular characterization of rotavirus species A (RVA) and to determine the frequency of RVC and RVH in fecal specimens from non-vaccinated piglets, across 5 farms in the metropolitan mesoregion of Belém, within the Amazon region, Brazil. In the previous study, porcine RVA was found in 9.9% (17/172) during the years 2008-2009. Using RT-PCR, Sanger nucleotide sequencing, and phylogenetic analyses, the entire genomes of 17 Brazilian porcine RVA strains were analyzed. The results indicated that all strains shared the same constellation, with the exception of the VP3 and NSP3 genes, which were M2 and T7 genotypes, respectively. The VP1, VP2, NSP2, NSP4 and NSP5 coding segments clustered into the Wa-like constellation; genotype 1, and VP7, VP4, VP6 and NSP1 genes were typically of porcine origin. RVA genes infecting pigs presented a high degree of nucleotide similarity to strains from other hosts, including humans. Little is known about the zoonotic potential of rotaviruses in Brazil, but the results of this study show dynamic interactions between human and porcine rotavirus strains and constitute an increase in knowledge on the diversity of RVA strains, in animal reservoirs circulating in the Amazon region.


Keywords: Porcine, Zoonotic potential, Rotavirus A, Phylogenetic analysis, Environmental microbiology
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How to cite this article:

Mayara, A.O. Neves, Daniel S. De Camargo, Kathleen V.S. de Araújo, Patrícia S. Lobo, Renato Da S. Bandeira, Luana S. Soares and Joana D.P. Mascarenhas. 2018. Genomic Analysis of Rotavirus Species A Isolated from Swine, Amazon Region, Brazil.Int.J.Curr.Microbiol.App.Sci. 7(11): 792-814. doi: https://doi.org/10.20546/ijcmas.2018.711.095