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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2022: 95.28
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28
For more details click here
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Original Research Articles                      Volume : 7, Issue:11, November, 2018
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38
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Int.J.Curr.Microbiol.App.Sci.2018.7(11): 781-791
DOI: https://doi.org/10.20546/ijcmas.2018.711.094


Molecular Characterization of Phytoplasma Associated with Crops, Weeds and Forest Trees in Andhra Pradesh, India
D. Vijay Kumar Naik1, B.V. Bhaskara Reddy2*, J. Sailaja Rani3, R. Sarada Jayalakshmi Devi1 and K.V. Hari Prasad4
1Department of Plant Pathology, S. V. Agricultural College Tirupati, India
2Department of Plant Pathology, IFT, Regional Agricultural Research Station, Tirupati, India
3Department of Plant Pathology, Agricultural College, Mahanandi, India
4Department of Entomology, S.V. Agricultural College, Tirupati, India
*Corresponding author
Abstract:

Totally 32 weed hosts and crop plants were collected and the DNA was isolated from 32 weeds and crops showing phytoplasma disease symptoms and subjected to PCR amplification with phytoplasma specific primers R16F2n/R16R2. The result shows that R16F2n/R16R2 primer amplified 1250bp product in 19 weeds and other crop species. The notable contribution in the present study was identification of six new hosts for phytoplasma for the first time in the Andhra Pradesh. They are Cleome gynendra, Solanum duclamara, Portulaca oleracea, Aerva lanata, Celosia argentea and Pergularia daemia in Andhra Pradesh.


Keywords: Phytoplasma 16S rDNA, Molecular characterization, Weeds and crops

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How to cite this article:

Vijay Kumar Naik, D., B.V. Bhaskara Reddy, J. Sailaja Rani, R. Sarada Jayalakshmi Devi and Hari Prasad, K.V. 2018. Molecular Characterization of Phytoplasma Associated with Crops, Weeds and Forest Trees in Andhra Pradesh, India.Int.J.Curr.Microbiol.App.Sci. 7(11): 781-791. doi: https://doi.org/10.20546/ijcmas.2018.711.094
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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