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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 781-791
DOI: https://doi.org/10.20546/ijcmas.2018.711.094


Molecular Characterization of Phytoplasma Associated with Crops, Weeds and Forest Trees in Andhra Pradesh, India
D. Vijay Kumar Naik1, B.V. Bhaskara Reddy2*, J. Sailaja Rani3, R. Sarada Jayalakshmi Devi1 and K.V. Hari Prasad4
1Department of Plant Pathology, S. V. Agricultural College Tirupati, India
2Department of Plant Pathology, IFT, Regional Agricultural Research Station, Tirupati, India
3Department of Plant Pathology, Agricultural College, Mahanandi, India
4Department of Entomology, S.V. Agricultural College, Tirupati, India
*Corresponding author
Abstract:

Totally 32 weed hosts and crop plants were collected and the DNA was isolated from 32 weeds and crops showing phytoplasma disease symptoms and subjected to PCR amplification with phytoplasma specific primers R16F2n/R16R2. The result shows that R16F2n/R16R2 primer amplified 1250bp product in 19 weeds and other crop species. The notable contribution in the present study was identification of six new hosts for phytoplasma for the first time in the Andhra Pradesh. They are Cleome gynendra, Solanum duclamara, Portulaca oleracea, Aerva lanata, Celosia argentea and Pergularia daemia in Andhra Pradesh.


Keywords: Phytoplasma 16S rDNA, Molecular characterization, Weeds and crops
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How to cite this article:

Vijay Kumar Naik, D., B.V. Bhaskara Reddy, J. Sailaja Rani, R. Sarada Jayalakshmi Devi and Hari Prasad, K.V. 2018. Molecular Characterization of Phytoplasma Associated with Crops, Weeds and Forest Trees in Andhra Pradesh, India.Int.J.Curr.Microbiol.App.Sci. 7(11): 781-791. doi: https://doi.org/10.20546/ijcmas.2018.711.094