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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:11, November, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 774-780
DOI: https://doi.org/10.20546/ijcmas.2018.711.093


Isolation, Characterization and Screening of Azospirillum from Tuberose Rhizoplane in Raichur
M. Avinash* and Mahadeva Swamy
Department of Agricultural Microbiology, University of Agricultural sciences, Raichur-584104, Karnataka, India
*Corresponding author
Abstract:

An experiment was carried out to isolate, characterize and screening of Azospirillum from tuberose rhizoplane. 40 bacterial strains were isolated showing characteristics of Azospirillum. Isolates were characterized based on utilization of glucose, biotin requirement, acid production in glucose peptone broth and denitrification test. Out of 40 isolates, 20 isolates required biotin for their growth and remaining 20 showed growth without biotin. 16 isolates showed positive for denitrification and they were tentatively identified as Azospirillum brasilense. All isolates produced acid and utilized glucose as carbon source. Isolates showed negative for denitrification test were screened through In vitro analysis.


Keywords: Azospirillum, Acid production, Biotin requirement, Denitrification, IAA, Nitrogen fixation

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How to cite this article:

Avinash, M. and Mahadeva Swamy. 2018. Isolation, Characterization and Screening of Azospirillum from Tuberose Rhizoplane in Raichur.Int.J.Curr.Microbiol.App.Sci. 7(11): 774-780. doi: https://doi.org/10.20546/ijcmas.2018.711.093
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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