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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 774-780
DOI: https://doi.org/10.20546/ijcmas.2018.711.093


Isolation, Characterization and Screening of Azospirillum from Tuberose Rhizoplane in Raichur
M. Avinash* and Mahadeva Swamy
Department of Agricultural Microbiology, University of Agricultural sciences, Raichur-584104, Karnataka, India
*Corresponding author
Abstract:

An experiment was carried out to isolate, characterize and screening of Azospirillum from tuberose rhizoplane. 40 bacterial strains were isolated showing characteristics of Azospirillum. Isolates were characterized based on utilization of glucose, biotin requirement, acid production in glucose peptone broth and denitrification test. Out of 40 isolates, 20 isolates required biotin for their growth and remaining 20 showed growth without biotin. 16 isolates showed positive for denitrification and they were tentatively identified as Azospirillum brasilense. All isolates produced acid and utilized glucose as carbon source. Isolates showed negative for denitrification test were screened through In vitro analysis.


Keywords: Azospirillum, Acid production, Biotin requirement, Denitrification, IAA, Nitrogen fixation
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How to cite this article:

Avinash, M. and Mahadeva Swamy. 2018. Isolation, Characterization and Screening of Azospirillum from Tuberose Rhizoplane in Raichur.Int.J.Curr.Microbiol.App.Sci. 7(11): 774-780. doi: https://doi.org/10.20546/ijcmas.2018.711.093