National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Systemic autoimmune diseases (SAD) are the diseases where multiple organs are involved in the presence of a large variety of auto antibodies directed against sub-cellular structures or molecules (E.g. nuclei, cytoplasm, mitochondria, DNA) and are characterized by presence of Antinuclear antibodies (ANA). Indirect immunofluorescence Assay (IIFA) on Hep-2 (human epithelial cell tumour line) is a classical technique for detection of ANA and is considered as “gold standard”. Though positive fluorescence pattern on IIFA indicates the presence of ANA, however it does not allow precise identification of these antibodies. For this specialized techniques like ELISA, western blotting or line immunoassay (LIA) are employed 1) To detect sensitivity and specificity of Line immuno assay (LIA) in comparison with Indirect immunofluorescence assay(IIFA) for the detection of anti-nuclear antibodies in diagnosis of systemic autoimmune disorders. A cross-sectional study was conducted and a total of 150 clinically suspected cases of SAD of both sexes and above 18yrs of age from various departments were included in the study and blood samples collected were subjected to Indirect Immunofluorescence test on Hep-2 cells coated slides and Line immunoassay. 150 samples were analyzed for ANA by IIFA and LIA. Out of 150 samples, 54 samples were positive by IIFA. Line immunoassay was positive in 49 samples. Sensitivity and Specificity of LIA was found to be 72.2% and 89.58% respectively. Positive Coincidence Rate came out to be 79.59%. In contrast to other studies, our study gave an apt correlation of ANA detection by Line Immuno Assay and indirect immunofluorescence assay.
Citations |
 |
 |
 |
 |
