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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 743-748
DOI: https://doi.org/10.20546/ijcmas.2018.711.089


Detection of Sensitivity and Specificity of Line Immuno Assay in Comparison with Indirect Immunofluorescence Assay for the Detection of Anti-Nuclear Antibodies in Diagnosis of Systemic Autoimmune Disorders
Jabeen Begum, V.V. Shailaja and Rajeshwar Rao
Department of Microbiology, Gandhi Medical College, Secunderabad, Telangana, India
*Corresponding author
Abstract:

Systemic autoimmune diseases (SAD) are the diseases where multiple organs are involved in the presence of a large variety of auto antibodies directed against sub-cellular structures or molecules (E.g. nuclei, cytoplasm, mitochondria, DNA) and are characterized by presence of Antinuclear antibodies (ANA). Indirect immunofluorescence Assay (IIFA) on Hep-2 (human epithelial cell tumour line) is a classical technique for detection of ANA and is considered as “gold standard”. Though positive fluorescence pattern on IIFA indicates the presence of ANA, however it does not allow precise identification of these antibodies. For this specialized techniques like ELISA, western blotting or line immunoassay (LIA) are employed 1) To detect sensitivity and specificity of Line immuno assay (LIA) in comparison with Indirect immunofluorescence assay(IIFA) for the detection of anti-nuclear antibodies in diagnosis of systemic autoimmune disorders. A cross-sectional study was conducted and a total of 150 clinically suspected cases of SAD of both sexes and above 18yrs of age from various departments were included in the study and blood samples collected were subjected to Indirect Immunofluorescence test on Hep-2 cells coated slides and Line immunoassay. 150 samples were analyzed for ANA by IIFA and LIA. Out of 150 samples, 54 samples were positive by IIFA. Line immunoassay was positive in 49 samples. Sensitivity and Specificity of LIA was found to be 72.2% and 89.58% respectively. Positive Coincidence Rate came out to be 79.59%. In contrast to other studies, our study gave an apt correlation of ANA detection by Line Immuno Assay and indirect immunofluorescence assay.


Keywords: Systemic Autoimmune diseases, Antinuclear antibody test, Indirect immunofluorescence Assay, Line Immunoassay
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How to cite this article:

Jabeen Begum, V.V. Shailaja and Rajeshwar Rao. 2018. Detection of Sensitivity and Specificity of Line Immuno Assay in Comparison with Indirect Immunofluorescence Assay for the Detection of Anti-Nuclear Antibodies in Diagnosis of Systemic Autoimmune Disorders.Int.J.Curr.Microbiol.App.Sci. 7(11): 743-748. doi: https://doi.org/10.20546/ijcmas.2018.711.089