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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 734-742
DOI: https://doi.org/10.20546/ijcmas.2018.711.088


Prevalence of Bud Blight of Tomato Caused by Groundnut bud necrosis virus in Tamil Nadu
M. Suganyadevi1*, S.K. Manoranjitham1, N. Senthil2, M. Raveendran2 and G.Karthikeyan1
1Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore-641003, Tamil Nadu, India
2Department of Plant Biotechnology, Tamil Nadu Agricultural University,
Coimbatore-641003, Tamil Nadu, India
*Corresponding author
Abstract:

A field survey was conducted in major tomato growing districts of Tamil Nadu viz., Coimbatore, Dharmapuri, Krishnagiri, Salem and Madurai during 2015-2016. Groundnut bud necrosis virus (GBNV) infection on tomato exhibited the symptoms viz., chlorotic and necrotic spots on leaves, necrosis of growing young buds (bud blight), bronzing, wilting and stunting. The per cent disease incidence of GBNV ranged from 18.6% to 35.3% in the above districts. A high per cent disease incidence of 35.3% was recorded in Thondamuthur village of Coimbatore district. Cowpea cv. CO5 was used as local lesion host for maintenance of GBNV isolates separately. A DNA fragment of around 800bp corresponding to a part of replicase gene was amplified using the tospovirus universal primer pair in all the samples. The DNA fragments were cloned into pGEMT vector and sequenced. The sequence analysis revealed higher similarity among the present isolates. However, they had an identity of 92.5% to 99.3% with the other isolates in NCBI. The results revealed that the isolates of GBNV is not divergent throughout Tamil Nadu.


Keywords: GBNV, Tomato, Tamil Nadu, Bud blight, Replicase protein gene, Orthotospovirus
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How to cite this article:

Suganyadevi, M., S.K. Manoranjitham, N. Senthil, M. Raveendran and Karthikeyan, G. 2018. Prevalence of Bud Blight of Tomato Caused by Groundnut bud necrosis virus in Tamil Nadu.Int.J.Curr.Microbiol.App.Sci. 7(11): 734-742. doi: https://doi.org/10.20546/ijcmas.2018.711.088