Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2018) [Effective from January 1, 2018]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
journals IJCMAS
See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 653-670
DOI: https://doi.org/10.20546/ijcmas.2018.711.079


Proteomic Analysis of a Compatible Interaction between Sorghum Downy Mildew Pathogen (Peronosclerospora sorghi) and Maize (Zea mays L.)
K.P. Jadhav1*, R. Veera Ranjani1, N. Senthil2, N. Arulkumar3, P.M. Tamilarasi5, K. Sumathi5, K.N. Ganesan5, V. Paranidharan4, M. Raveendran1, Gon Sup Kim3 and J. Ramalingam1
1Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu, India
2Department of Biotechnology, Agriculture College & Research Institute, Tamil Nadu Agricultural University, Madurai - 625 104, Tamil Nadu, India
3Research Institute of Life Science and College of Veterinary Medicine Gyeongsang National University Jinju, Gyeongnam - 660 701, South Korea
4Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu, India
5Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu, India
*Corresponding author
Abstract:

Sorghum downy mildew (SDM) caused by the oomycete, Peronosclerospora sorghi, is one of the destructive diseases that afflict maize. P. sorghi infects a susceptible host, hindering its growth and altering its morphology. To understand the molecular basis of the compatibility interaction between P. sorghi and maize, a comparative proteomic approach (2D-PAGE) was employed between the mock-inoculated (control) and SDM-inoculated leaves in the susceptible genotypes of maize (UMI79 and CM500). Seventeen spots showed a significant difference in the abundance of proteins in control and inoculated samples were further analyzed with MALDI-TOF/MS. The resulting peptide mass fingerprint was subjected to MASCOT analysis and it was found that most were related to stress that includes lipoxygenase and DEAD-box ATP-dependent RNA helicases, microtubule-associated protein and probable protein disulphide isomerase. Additionally, proteins involved in the cell cycle/endoreplication such as DNA topoisomerase 6 subunit A and retinoblastoma-related proteins were differentially expressed during infection. The possible roles of these proteins in response to P. sorghi infection in maize are discussed.


Keywords: Maize, Sorghum downy mildew, 2D-PAGE, MALDI-TOF, Lipoxygenase, Cell cycle
Download this article as Download

How to cite this article:

Jadhav, K.P., R. Veera Ranjani, N. Senthil, N. Arulkumar, P.M. Tamilarasi, K. Sumathi, K.N. Ganesan, V. Paranidharan, M. Raveendran, Gon Sup Kim and Ramalingam, J. 2018. Proteomic Analysis of a Compatible Interaction between Sorghum Downy Mildew Pathogen (Peronosclerospora sorghi) and Maize (Zea mays L.).Int.J.Curr.Microbiol.App.Sci. 7(11): 653-670. doi: https://doi.org/10.20546/ijcmas.2018.711.079