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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 373-379
DOI: https://doi.org/10.20546/ijcmas.2018.711.046


ITS rDNA Analyses in the Identification and Differentiation of Isolates of Fusarium oxysporum f. sp. ciceri Causing Chickpea Wilt
K.L. Nandeesha, Shalini N. Huilgol and M.D. Patil
Department of Plant Pathology, College of Agriculture, Vijayapura, India
*Corresponding author
Abstract:

Eight variants of Fusarium oxysporum f. sp. ciceri were isolated from wilt infected chickpea plants from diverse locations of northern Karnataka. Genetic variability was studied by polymerase chain reaction (PCR) amplification with specific primer and genetic identity among each variant was calculated and results depicted that there was minimum genetic variation among the variants collected from the diverse locations. At 0.01 similarity coefficient the variants separated into two clusters. Cluster A contained six variants viz., Foc V, Foc Ku, Foc B, Foc S, Foc Ka and Foc H and cluster B contained two variants Foc R and Foc J.


Keywords: Fusarium oxysporum f. sp. ciceri chickpea wilt, PCR, ITS
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How to cite this article:

Nandeesha, K.L., Shalini N. Huilgol and Patil, M.D. 2018. ITS rDNA Analyses in the Identification and Differentiation of Isolates of Fusarium oxysporum f. sp. ciceri Causing Chickpea Wilt.Int.J.Curr.Microbiol.App.Sci. 7(11): 373-379. doi: https://doi.org/10.20546/ijcmas.2018.711.046