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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 51-64
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.006


Extracellular Hydrolases producing Haloarchaea from Marine Salterns at Okhamadhi, Gujarat, India
Bhavini N. Rathod1, Harshil H. Bhatt2 and Vivek N. Upasani1*
1Department of   Microbiology, M.G. Science Institute, Ahmedabad- 380009, India
2Kadi Sarva Vishwavidyalay Gandhinagar-23, India
*Corresponding author
Abstract:

Haloarchaea thrive in hypersaline environments such as marine salterns, saline soils, soda lakes, salted foods, etc. The lysis of marine phyto- and zoo-planktons such as algae, diatoms, shrimps, purple and green bacteria, fish, etc. releases biopolymers namely cellulose, starch, chitin, proteins, lipids, etc. in the saline ecosystems. The chemorganotrophic haloarchaea therefore, need to produce hydrolytic enzymes to utilize these substrates. However, the raw solar salt used for preservation can cause spoilage of foods due to the growth of halobacteria leading to economic loss. We report here the isolation and identification of extracellular hydrolases (substrates casein, gelatin, starch, and Tweens: 20, 60, 40, 80) producing haloarchaea isolated from the salt and brine samples collected from marine salterns at Okhamadhi, Gujarat, India. Morphological, physiological, detection of diether lipids, carotenoids, antibiotic sensitivity and molecular (16S rRNA) sequencing was carriedout. Majority of the isolates showed their potential to hydrolyze at least one substrate, while one strain BVM005 belonging to the genus Haloferax showed multiple hydrolytic activities against four substrates (casein, gelatin, starch and Tween 80).The 16S rRNA sequences of these strains were deposited in the GenBank, accession numbers: KP636732-33, 34, 35, 36, and 37. This is the first paper concerning the identification of hydrolases producing haloarchaea based on 16S rRNA sequencing from Okhamadhi, Gujarat.


Keywords: Extremozymes, haloarchaea, hydrolases, Halobacterium, Haloferax, Halopetinus, Okhamadhi
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How to cite this article:

Bhavini N. Rathod, Harshil H. Bhatt and Vivek N. Upasani. 2016. Extracellular Hydrolases producing Haloarchaea from Marine Salterns at Okhamadhi, Gujarat, India.Int.J.Curr.Microbiol.App.Sci. 5(11): 51-64. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.006