Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2018) [Effective from January 1, 2018]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
journals IJCMAS
See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 1177-1187
DOI: https://doi.org/10.20546/ijcmas.2018.711.137


Isolation and Identification of Listeria monocytogenes from Fish Intestines and Phylogenetic Analysis
N. Madharsha1, Veeramanikandan Veeramani1*, Jaianand Kannaiyan2 and Balaji Paulraj2
1Department of Microbiology
2Department of Biotechnology, MGR College, Dr. MGR Nagar, Hosur, Tamil Nadu, India
*Corresponding author
Abstract:

Forty fish procured from retail shops located in and around from west Chennai were examined for the presence of Listeria monocytogenes. The cultural, biochemical, molecular and phylogenetic relationship characterization of isolates was performed to detect genetic variations among L. monocytogenes isolates. The selective enrichment procedure followed by selective isolation was used for listeria isolation from fish intestines. Twenty (50%) L. monocytogenes were isolated and identified from fish intestines. Two different varieties were analyzed by the BLAST sequencing analysis of the isolates. The results indicated that the isolates might have originated from different sources, and fish contaminated with L. monocytogenes may cause serious health problems in humans.


Keywords: Listeriosis, Listeria monocytogenes, Labeo catla (Catla fish), Dasyatis pastinaca (Stingrays fish), Phylogenetic analysis
Download this article as Download

How to cite this article:

Madharsha, N., Veeramanikandan Veeramani, Jaianand Kannaiyan and Balaji Paulraj. 2018. Isolation and Identification of Listeria monocytogenes from Fish Intestines and Phylogenetic Analysis.Int.J.Curr.Microbiol.App.Sci. 7(11): 1177-1187. doi: https://doi.org/10.20546/ijcmas.2018.711.137